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Sample GSM1193380 Query DataSets for GSM1193380
Status Public on Jun 30, 2014
Title Spontaneous IPF, Replicate 14
Sample type RNA
 
Source name Alveolar macrophage isolated from Spontaneous IPF
Organism Homo sapiens
Characteristics diagnosis: Spontaneous Idiopathic Pulmonary Fibrosis (IPF)
tissue: Alveolar Macrophage
Extracted molecule total RNA
Extraction protocol Total RNA extraction.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 ug total RNA (Affymetrix Expression Analysis Technical Manual, 2005, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using the Affymetrix GeneArray scanner.
Data processing The data were analyzed with Affymetrix apt version 1.12, GeneSpring 11.5.1 . Apt was used to determine present/absent calls and GeneSpring RMA normalization was applied.
 
Submission date Jul 22, 2013
Last update date Sep 01, 2016
Contact name Eric Billings
Organization name NHLBI
Street address 10 Center Drive
City Bethesda
State/province MD
ZIP/Postal code 20874
Country USA
 
Platform ID GPL96
Series (1)
GSE49072 Alveolar Macrophage Gene Expression in Human Pulmonary Fibrosis
Relations
Reanalyzed by GSE86363

Data table header descriptions
ID_REF
VALUE RMA normalized expression

Data table
ID_REF VALUE
AFFX-BioB-5_at 5.048331
AFFX-BioB-M_at 4.9131002
AFFX-BioB-3_at 5.625728
AFFX-BioC-5_at 4.676612
AFFX-BioC-3_at 4.549736
AFFX-BioDn-5_at 4.9506683
AFFX-BioDn-3_at 2.6408892
AFFX-CreX-5_at 2.437192
AFFX-CreX-3_at 1.9426336
AFFX-DapX-5_at 0.40553474
AFFX-DapX-M_at 1.1020498
AFFX-DapX-3_at 0.71530056
AFFX-LysX-5_at 1.3051481
AFFX-LysX-M_at 0.26007652
AFFX-LysX-3_at 1.764368
AFFX-PheX-5_at 0.67658377
AFFX-PheX-M_at 0.26831007
AFFX-PheX-3_at 0.34729958
AFFX-ThrX-5_at -0.3748803
AFFX-ThrX-M_at 0.26586628

Total number of rows: 22283

Table truncated, full table size 478 Kbytes.




Supplementary file Size Download File type/resource
GSM1193380_RIPF-91A.CEL.gz 2.8 Mb (ftp)(http) CEL
Processed data included within Sample table

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