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Sample GSM1195692 Query DataSets for GSM1195692
Status Public on Jan 23, 2015
Title DU145 xenograft in NOD/SCID mice_NVP-BEZ235_4 weeks
Sample type RNA
 
Source name DU145 xenograft in NOD/SCID mice_NVP-BEZ235_4 weeks
Organism Homo sapiens
Characteristics cell type: DU145 xenograft in NOD/SCID mice
dose: 12.5 mg/kg, perorally
schedule: once per day
Growth protocol DU145 xenograft tumors were established by using early-passage cells and maintained in NOD.CB17-Prkdc(SCID) mice. For s.c. tumor development, 100 μL of collagen-embedded cells was injected s.c. into 5- to 8-week-old NOD.CB17-Prkdc(SCID) mice. The mice were observed for 3–5 months for appearance and development of tumors.
Extracted molecule total RNA
Extraction protocol Qiagen RNeasy extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 500 ng total RNA (Affymetrix GeneChip Expression Manual 701021 Rev.5)
 
Hybridization protocol Following fragmentation, 12.5 ug of cRNA were hybridized for 16 hr at 45C on HG_U133_2 Arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the Affymetrix GeneChip Scanner 3000
Data processing CEL files were processed using GCRMA in R (version 2.6.2 (2008-02-08)), using packages downloaded from Bioconductor
 
Submission date Jul 25, 2013
Last update date Jan 24, 2015
Contact name John R Walker
E-mail(s) jwalker@gnf.org
Phone 858-812-1636
Organization name Genomics Institute of the Novartis Research Foundation
Lab Genetics Core
Street address 10675 John Jay Hopkins
City San Diego
State/province CA
ZIP/Postal code 92121
Country USA
 
Platform ID GPL570
Series (1)
GSE49232 Gene expression analysis of in vivo-grown tumors treated with compounds that either de-bulk the tumor or target cancer stem cells

Data table header descriptions
ID_REF
VALUE Gcrma signal was unlogged

Data table
ID_REF VALUE
1007_s_at 1488.6
1053_at 1501.317
117_at 22.981
121_at 25.305
1255_g_at 4.642
1294_at 4.357
1316_at 13.193
1320_at 5.984
1405_i_at 27.948
1431_at 7.12
1438_at 4.448
1487_at 362.791
1494_f_at 4.132
1552256_a_at 4142.51
1552257_a_at 966.736
1552258_at 5.6
1552261_at 6.21
1552263_at 111.166
1552264_a_at 249.107
1552266_at 8.08

Total number of rows: 54675

Table truncated, full table size 937 Kbytes.




Supplementary file Size Download File type/resource
GSM1195692_3PVR09012703.CEL.gz 8.2 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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