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Sample GSM1195729 Query DataSets for GSM1195729
Status Public on Jul 26, 2013
Title Monocytes (SAMPLE 2)
Sample type RNA
Source name monocytes
Organism Homo sapiens
Characteristics cell type: monocytes
culture condition: Monocytes directly after isolation
Treatment protocol cells were stimulated with indicated cytokines for 5 days.
Growth protocol Monocytes were isolated from buffy coats of healthy blood donors by lymphoprep density gradient centrifugation followed by plastic adherence and culture in IMDM containing 10% human pool serum, 1% penicillin/streptomycin (Invitrogen, San Diego, CA), and 0.25% ciproxin (Bayer, Leverkusen, Germany).
Extracted molecule total RNA
Extraction protocol Tripure isolation reagent ( roche) + Rneasy cleanup
Label biotin
Label protocol Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
Hybridization protocol Standard Illumina hybridization protocol
Scan protocol Standard Illumina scanning protocol
Description Replicate 2
Data processing Quantile normalized
Submission date Jul 26, 2013
Last update date Apr 12, 2018
Contact name Fernando O Martinez Estrada
Organization name University of Surrey
Department Faculty of Health & Medical Sciences
Lab Innate Immunity
Street address Stag Hill
City Guildford
State/province Surrey
ZIP/Postal code GU2 7XH
Country United Kingdom
Platform ID GPL10558
Series (2)
GSE35495 Genome-wide analysis of human and mouse macrophages, resting and activated
GSE49240 Similarities and differences between human activated macrophages I

Data table header descriptions
VALUE RMA signal
Detection Pval

Data table
ID_REF VALUE Detection Pval
ILMN_1762337 5.787190692 0.5987
ILMN_2055271 5.946926027 0.87013
ILMN_1736007 5.571475974 0.10649
ILMN_2383229 5.655844969 0.23506
ILMN_1806310 5.66443097 0.25325
ILMN_1779670 5.813263213 0.65325
ILMN_1653355 5.979562337 0.91429
ILMN_1717783 5.616701862 0.17403
ILMN_1705025 5.808178573 0.64416
ILMN_1814316 5.746179286 0.46753
ILMN_2359168 5.598654438 0.14675
ILMN_1731507 5.576867501 0.11818
ILMN_1787689 5.777005873 0.56364
ILMN_3241953 5.689863134 0.32208
ILMN_1745607 5.75855259 0.5
ILMN_2136495 5.54426839 0.07403
ILMN_1668111 5.724719283 0.40779
ILMN_2295559 5.792668019 0.61688
ILMN_1735045 7.899631959 1
ILMN_1680754 5.946926027 0.87273

Total number of rows: 47311

Table truncated, full table size 1443 Kbytes.

Supplementary data files not provided
Raw data are available on Series record
Processed data included within Sample table

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