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Sample GSM1195731 Query DataSets for GSM1195731
Status Public on Jul 26, 2013
Title Medium (SAMPLE 4)
Sample type RNA
Source name monocyte-derived macrophages, Medium
Organism Homo sapiens
Characteristics cell type: monocyte-derived macrophages
culture condition: 10% Human serum
Treatment protocol cells were stimulated with indicated cytokines for 5 days.
Growth protocol Monocytes were isolated from buffy coats of healthy blood donors by lymphoprep density gradient centrifugation followed by plastic adherence and culture in IMDM containing 10% human pool serum, 1% penicillin/streptomycin (Invitrogen, San Diego, CA), and 0.25% ciproxin (Bayer, Leverkusen, Germany).
Extracted molecule total RNA
Extraction protocol Tripure isolation reagent ( roche) + Rneasy cleanup
Label biotin
Label protocol Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
Hybridization protocol Standard Illumina hybridization protocol
Scan protocol Standard Illumina scanning protocol
Description Replicate 2
Data processing Quantile normalized
Submission date Jul 26, 2013
Last update date Apr 12, 2018
Contact name Fernando O Martinez Estrada
Organization name University of Surrey
Department Faculty of Health & Medical Sciences
Lab Innate Immunity
Street address Stag Hill
City Guildford
State/province Surrey
ZIP/Postal code GU2 7XH
Country United Kingdom
Platform ID GPL10558
Series (2)
GSE35495 Genome-wide analysis of human and mouse macrophages, resting and activated
GSE49240 Similarities and differences between human activated macrophages I

Data table header descriptions
VALUE RMA signal
Detection Pval

Data table
ID_REF VALUE Detection Pval
ILMN_1762337 5.90382177 0.84026
ILMN_2055271 5.967941468 0.92987
ILMN_1736007 5.688625644 0.32987
ILMN_2383229 5.648104648 0.24416
ILMN_1806310 5.717218661 0.40779
ILMN_1779670 5.725243883 0.42727
ILMN_1653355 5.851373302 0.74416
ILMN_1717783 5.48167037 0.03636
ILMN_1705025 5.901360313 0.83636
ILMN_1814316 5.878680776 0.8039
ILMN_2359168 5.761121251 0.51558
ILMN_1731507 5.450635624 0.01558
ILMN_1787689 5.838779104 0.71169
ILMN_3241953 6.712908405 1
ILMN_1745607 9.938274167 1
ILMN_2136495 5.49529925 0.04156
ILMN_1668111 5.642346065 0.22857
ILMN_2295559 5.703133938 0.36494
ILMN_1735045 6.612775185 1
ILMN_1680754 5.89643824 0.83117

Total number of rows: 47311

Table truncated, full table size 1439 Kbytes.

Supplementary data files not provided
Raw data are available on Series record
Processed data included within Sample table

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