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Sample GSM1195732 Query DataSets for GSM1195732
Status Public on Jul 26, 2013
Title M-CSF (SAMPLE 5)
Sample type RNA
 
Source name monocyte-derived macrophages, M-CSF
Organism Homo sapiens
Characteristics cell type: monocyte-derived macrophages
culture condition: 10% Human serum+50 ng/ml of M-CSF
Treatment protocol cells were stimulated with indicated cytokines for 5 days.
Growth protocol Monocytes were isolated from buffy coats of healthy blood donors by lymphoprep density gradient centrifugation followed by plastic adherence and culture in IMDM containing 10% human pool serum, 1% penicillin/streptomycin (Invitrogen, San Diego, CA), and 0.25% ciproxin (Bayer, Leverkusen, Germany).
Extracted molecule total RNA
Extraction protocol Tripure isolation reagent ( roche) + Rneasy cleanup
Label biotin
Label protocol Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
 
Hybridization protocol Standard Illumina hybridization protocol
Scan protocol Standard Illumina scanning protocol
Description Replicate 1
Data processing Quantile normalized
 
Submission date Jul 26, 2013
Last update date Apr 12, 2018
Contact name Fernando O Martinez Estrada
E-mail(s) f.martinezestrada@surrey.ac.uk
Organization name University of Surrey
Department Faculty of Health & Medical Sciences
Lab Innate Immunity
Street address Stag Hill
City Guildford
State/province Surrey
ZIP/Postal code GU2 7XH
Country United Kingdom
 
Platform ID GPL10558
Series (2)
GSE35495 Genome-wide analysis of human and mouse macrophages, resting and activated
GSE49240 Similarities and differences between human activated macrophages I

Data table header descriptions
ID_REF
VALUE RMA signal
Detection Pval

Data table
ID_REF VALUE Detection Pval
ILMN_1762337 5.875788181 0.82468
ILMN_2055271 5.929688525 0.9039
ILMN_1736007 5.791617441 0.62597
ILMN_2383229 5.705364893 0.37922
ILMN_1806310 5.833450266 0.72857
ILMN_1779670 5.715635518 0.3987
ILMN_1653355 6.07151586 0.98182
ILMN_1717783 5.549524448 0.08052
ILMN_1705025 5.8089302 0.66753
ILMN_1814316 5.8432386 0.75844
ILMN_2359168 5.546679088 0.07792
ILMN_1731507 5.448494316 0.01818
ILMN_1787689 5.799281062 0.64545
ILMN_3241953 6.728430809 1
ILMN_1745607 9.430609814 1
ILMN_2136495 5.474700797 0.02597
ILMN_1668111 5.546679088 0.07792
ILMN_2295559 5.77201029 0.55974
ILMN_1735045 5.78410293 0.60519
ILMN_1680754 5.969578417 0.94545

Total number of rows: 47311

Table truncated, full table size 1437 Kbytes.




Supplementary data files not provided
Raw data are available on Series record
Processed data included within Sample table

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