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Sample GSM1195738 Query DataSets for GSM1195738
Status Public on Jul 26, 2013
Title IFN-y (SAMPLE 11)
Sample type RNA
 
Source name monocyte-derived macrophages, IFN-y
Organism Homo sapiens
Characteristics cell type: monocyte-derived macrophages
culture condition: 10% Human serum+50 U/ml of IFN-γ plus 12.5 ng/ml of TNF-α
Treatment protocol cells were stimulated with indicated cytokines for 5 days.
Growth protocol Monocytes were isolated from buffy coats of healthy blood donors by lymphoprep density gradient centrifugation followed by plastic adherence and culture in IMDM containing 10% human pool serum, 1% penicillin/streptomycin (Invitrogen, San Diego, CA), and 0.25% ciproxin (Bayer, Leverkusen, Germany).
Extracted molecule total RNA
Extraction protocol Tripure isolation reagent ( roche) + Rneasy cleanup
Label biotin
Label protocol Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
 
Hybridization protocol Standard Illumina hybridization protocol
Scan protocol Standard Illumina scanning protocol
Description Replicate 1
Data processing Quantile normalized
 
Submission date Jul 26, 2013
Last update date Apr 12, 2018
Contact name Fernando O Martinez Estrada
E-mail(s) f.martinezestrada@surrey.ac.uk
Organization name University of Surrey
Department Faculty of Health & Medical Sciences
Lab Innate Immunity
Street address Stag Hill
City Guildford
State/province Surrey
ZIP/Postal code GU2 7XH
Country United Kingdom
 
Platform ID GPL10558
Series (2)
GSE35495 Genome-wide analysis of human and mouse macrophages, resting and activated
GSE49240 Similarities and differences between human activated macrophages I

Data table header descriptions
ID_REF
VALUE RMA signal
Detection Pval

Data table
ID_REF VALUE Detection Pval
ILMN_1762337 5.858623534 0.78831
ILMN_2055271 5.917090222 0.88701
ILMN_1736007 5.712883574 0.41558
ILMN_2383229 5.866107101 0.81039
ILMN_1806310 5.856109658 0.78442
ILMN_1779670 5.744279021 0.50649
ILMN_1653355 5.924470715 0.8987
ILMN_1717783 5.454647464 0.02468
ILMN_1705025 6.023963822 0.97662
ILMN_1814316 5.92667388 0.8987
ILMN_2359168 5.540966419 0.08442
ILMN_1731507 5.432700697 0.01558
ILMN_1787689 5.639938483 0.24026
ILMN_3241953 6.569399871 0.9987
ILMN_1745607 7.607314248 1
ILMN_2136495 5.562509963 0.10909
ILMN_1668111 5.8209109 0.71039
ILMN_2295559 5.636829444 0.23636
ILMN_1735045 5.62782962 0.22208
ILMN_1680754 5.841219742 0.75325

Total number of rows: 47311

Table truncated, full table size 1449 Kbytes.




Supplementary data files not provided
Raw data are available on Series record
Processed data included within Sample table

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