|
| Status |
Public on Jul 26, 2013 |
| Title |
IFN-y (SAMPLE 12) |
| Sample type |
RNA |
| |
|
| Source name |
monocyte-derived macrophages, IFN-y
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: monocyte-derived macrophages
culture condition: 10% Human serum+50 U/ml of IFN-γ plus 12.5 ng/ml of TNF-α
|
| Treatment protocol |
cells were stimulated with indicated cytokines for 5 days.
|
| Growth protocol |
Monocytes were isolated from buffy coats of healthy blood donors by lymphoprep density gradient centrifugation followed by plastic adherence and culture in IMDM containing 10% human pool serum, 1% penicillin/streptomycin (Invitrogen, San Diego, CA), and 0.25% ciproxin (Bayer, Leverkusen, Germany).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Tripure isolation reagent ( roche) + Rneasy cleanup
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
Replicate 2
|
| Data processing |
Quantile normalized
|
| |
|
| Submission date |
Jul 26, 2013 |
| Last update date |
Apr 12, 2018 |
| Contact name |
Fernando O Martinez Estrada |
| E-mail(s) |
f.martinezestrada@surrey.ac.uk
|
| Organization name |
University of Surrey
|
| Department |
Faculty of Health & Medical Sciences
|
| Lab |
Innate Immunity
|
| Street address |
Stag Hill
|
| City |
Guildford |
| State/province |
Surrey |
| ZIP/Postal code |
GU2 7XH |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL10558 |
| Series (2) |
| GSE35495 |
Genome-wide analysis of human and mouse macrophages, resting and activated |
| GSE49240 |
Similarities and differences between human activated macrophages I |
|
