|
| Status |
Public on Jul 30, 2013 |
| Title |
ky13 Replicate 2 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
Reference (mixed stage, mixed N2:CB4856)
|
| Organism |
Caenorhabditis elegans |
| Characteristics |
tissue: whole worm
strain: reference (mixed stage, mixed N2:CB4856)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using Trizol following manufacturer's instructions
|
| Label |
Cy3
|
| Label protocol |
RNA was labeled using the Low RNA Input Fluorescent Linear Amplification Kit (Agilent, San Jose CA) according to the manufacturer's instructions
|
| |
|
| Channel 2 |
| Source name |
ky13
|
| Organism |
Caenorhabditis elegans |
| Characteristics |
strain: ky13
genetic background/npr-1 allele: N2 (putative null allele)
tissue: whole worm
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using Trizol following manufacturer's instructions
|
| Label |
Cy5
|
| Label protocol |
RNA was labeled using the Low RNA Input Fluorescent Linear Amplification Kit (Agilent, San Jose CA) according to the manufacturer's instructions
|
| |
|
| |
| Hybridization protocol |
Oligoarray control targets and hybridization buffer (Agilent In Situ Hybridization Kit Plus) were added, and samples were applied to microarrays enclosed in Agilent SureHyb-enabled hybridization chambers. After hybridization, slides were washed sequential
|
| Scan protocol |
Scanned on an Agilent G2505B Scanner
|
| Description |
ky13 Biological Replicate 2
|
| Data processing |
Median signal processed in R using limma, background corrected, within array loess normalization, and Gquantile between array normalization, replicate probes averaged
|
| |
|
| Submission date |
Jul 29, 2013 |
| Last update date |
Jul 30, 2013 |
| Contact name |
Leonid Kruglyak |
| Organization name |
Princeton University
|
| Street address |
110 Carl Ichan Laboratory
|
| City |
Princeton |
| State/province |
NJ |
| ZIP/Postal code |
08540 |
| Country |
USA |
| |
|
| Platform ID |
GPL8209 |
| Series (1) |
| GSE49307 |
A variant in the neuropeptide receptor npr-1 is a major determinant of Caenorhabditis elegans growth and physiology |
|
