|
Status |
Public on Jul 30, 2013 |
Title |
kyIR9 Replicate 2 |
Sample type |
RNA |
|
|
Channel 1 |
Source name |
Reference (mixed stage, mixed N2:CB4856)
|
Organism |
Caenorhabditis elegans |
Characteristics |
tissue: whole worm strain: reference (mixed stage, mixed N2:CB4856)
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA extracted using Trizol following manufacturer's instructions
|
Label |
Cy3
|
Label protocol |
RNA was labeled using the Low RNA Input Fluorescent Linear Amplification Kit (Agilent, San Jose CA) according to the manufacturer's instructions
|
|
|
Channel 2 |
Source name |
kyIR9
|
Organism |
Caenorhabditis elegans |
Characteristics |
strain: kyIR9 genetic background/npr-1 allele: CB4856 (N2 introgression) tissue: whole worm
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA extracted using Trizol following manufacturer's instructions
|
Label |
Cy5
|
Label protocol |
RNA was labeled using the Low RNA Input Fluorescent Linear Amplification Kit (Agilent, San Jose CA) according to the manufacturer's instructions
|
|
|
|
Hybridization protocol |
Oligoarray control targets and hybridization buffer (Agilent In Situ Hybridization Kit Plus) were added, and samples were applied to microarrays enclosed in Agilent SureHyb-enabled hybridization chambers. After hybridization, slides were washed sequential
|
Scan protocol |
Scanned on an Agilent G2505B Scanner
|
Description |
kyIR9 Biological Replicate 2
|
Data processing |
Median signal processed in R using limma, background corrected, within array loess normalization, and Gquantile between array normalization, replicate probes averaged
|
|
|
Submission date |
Jul 29, 2013 |
Last update date |
Jul 30, 2013 |
Contact name |
Leonid Kruglyak |
Organization name |
Princeton University
|
Street address |
110 Carl Ichan Laboratory
|
City |
Princeton |
State/province |
NJ |
ZIP/Postal code |
08540 |
Country |
USA |
|
|
Platform ID |
GPL8209 |
Series (1) |
GSE49307 |
A variant in the neuropeptide receptor npr-1 is a major determinant of Caenorhabditis elegans growth and physiology |
|