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Sample GSM1197093 Query DataSets for GSM1197093
Status Public on Jul 30, 2013
Title kyIR9 Replicate 2
Sample type RNA
 
Channel 1
Source name Reference (mixed stage, mixed N2:CB4856)
Organism Caenorhabditis elegans
Characteristics tissue: whole worm
strain: reference (mixed stage, mixed N2:CB4856)
Extracted molecule total RNA
Extraction protocol Total RNA extracted using Trizol following manufacturer's instructions
Label Cy3
Label protocol RNA was labeled using the Low RNA Input Fluorescent Linear Amplification Kit (Agilent, San Jose CA) according to the manufacturer's instructions
 
Channel 2
Source name kyIR9
Organism Caenorhabditis elegans
Characteristics strain: kyIR9
genetic background/npr-1 allele: CB4856 (N2 introgression)
tissue: whole worm
Extracted molecule total RNA
Extraction protocol Total RNA extracted using Trizol following manufacturer's instructions
Label Cy5
Label protocol RNA was labeled using the Low RNA Input Fluorescent Linear Amplification Kit (Agilent, San Jose CA) according to the manufacturer's instructions
 
 
Hybridization protocol Oligoarray control targets and hybridization buffer (Agilent In Situ Hybridization Kit Plus) were added, and samples were applied to microarrays enclosed in Agilent SureHyb-enabled hybridization chambers. After hybridization, slides were washed sequential
Scan protocol Scanned on an Agilent G2505B Scanner
Description kyIR9 Biological Replicate 2
Data processing Median signal processed in R using limma, background corrected, within array loess normalization, and Gquantile between array normalization, replicate probes averaged
 
Submission date Jul 29, 2013
Last update date Jul 30, 2013
Contact name Leonid Kruglyak
Organization name Princeton University
Street address 110 Carl Ichan Laboratory
City Princeton
State/province NJ
ZIP/Postal code 08540
Country USA
 
Platform ID GPL8209
Series (1)
GSE49307 A variant in the neuropeptide receptor npr-1 is a major determinant of Caenorhabditis elegans growth and physiology

Data table header descriptions
ID_REF
VALUE log2(Cy5/Cy3)

Data table
ID_REF VALUE
A_12_P108045 0.212856757
A_12_P108879 0.135888628
A_12_P115540 -0.60035568
A_12_P101622 NULL
A_12_P105685 -0.678195968
A_12_P117297 -0.884642285
A_12_P104093 0.102675631
A_12_P120371 0.66792663
A_12_P117675 -1.50852618
A_12_P114854 0.660231205
A_12_P119331 0.348094487
A_12_P101256 NULL
A_12_P119545 -0.422255567
A_12_P118044 NULL
A_12_P120220 0.127758195
A_12_P119449 0.043090289
A_12_P105363 0.396529154
A_12_P107391 0.260756949
A_12_P119234 -0.693093574
A_12_P106869 NULL

Total number of rows: 20401

Table truncated, full table size 474 Kbytes.




Supplementary file Size Download File type/resource
GSM1197093_kyIR9_3_7.txt.gz 13.3 Mb (ftp)(http) TXT
Processed data included within Sample table

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