|
| Status |
Public on Jul 30, 2013 |
| Title |
left EC, biological rep3 |
| Sample type |
RNA |
| |
|
| Source name |
left entorhinal cortex of a 6 month-old C57BL/6 wild-type mouse
|
| Organism |
Mus musculus |
| Characteristics |
tissue: left entorhinal cortex
genotype: wild-type
age: 6 month
strain: C57BL/6
|
| Treatment protocol |
Mice were not subjected to any treatment.
|
| Growth protocol |
Mice were kept in the animal facility in a 12/12 hour light/dark cycle.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Dissected lef and right cortical samples were immediately processed for RNA extraction by using the RNeasy Kit from QIAGEN according to manufacturer’s instructions. Before running the microarrays, RNA quality and integrity was monitored on an Agilent BioAnalyzer.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Genechip Mouse gene 2.0 ST arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
Gene expression data from left mouse EC
|
| Data processing |
Microarrays data were analyzed through the use of Ingenuity® iReport (Ingenuity® Systems, www.ingenuity.com). Please see attached file with the list of genes ordered according to their fold change and p values.
|
| |
|
| Submission date |
Jul 29, 2013 |
| Last update date |
Jul 30, 2013 |
| Contact name |
Diego E Berman |
| E-mail(s) |
db2146@columbia.edu
|
| Organization name |
Columbia University
|
| Department |
Pathology and Cell Biology
|
| Street address |
630 W 168th St. #12-510
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10032 |
| Country |
USA |
| |
|
| Platform ID |
GPL16570 |
| Series (1) |
| GSE49311 |
Expression data from left versus right mouse entorhinal cortex (EC). |
|
