|
| Status |
Public on Feb 04, 2014 |
| Title |
61 |
| Sample type |
RNA |
| |
|
| Source name |
MDA-MB-468 ALDH1A3 shRNA1 cells
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: MDA-MB-468
cell type: breast cancer
|
| Growth protocol |
Stable transfectants were maintained in DMEM supplemented with 10% FBS and 0.25ug/mL puromycin.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA extracted with TRIzol and Purelink RNA kit
|
| Label |
CY3
|
| Label protocol |
400ng of the RNA samples spiked with RNA spike in controls were converted to cDNA and in vitro transcribed for amplification and incorporation of labelled CTP (cRNA).
|
| |
|
| Hybridization protocol |
Purified cRNA samples were fragmented and hybridized to an Agilent SurePrint G3 8x60K Gene Expression microarray v2 slide
|
| Scan protocol |
scanned at 5uM resolution with an Agilent G2565CA High Resolution Scanner
|
| Description |
puromycin selected polyclonal cells
|
| Data processing |
Data was processed with Agilent’s Feature Extraction software version 11.0.1.1 using the protocol GE1_1100_Jul11 and 8x60K grid file 039494_D_F_20120628. Samples were normalized using the percentile shift algorithm with no baseline transformation.
|
| |
|
| Submission date |
Jul 29, 2013 |
| Last update date |
Feb 04, 2014 |
| Contact name |
Paola Marcato |
| E-mail(s) |
paola.marcato@dal.ca
|
| Phone |
9024944239
|
| Organization name |
Dalhousie University
|
| Department |
Pathology
|
| Street address |
11F11, 5850 College Street
|
| City |
Halifax |
| State/province |
Nova Scotia |
| ZIP/Postal code |
B3H 4R2 |
| Country |
Canada |
| |
|
| Platform ID |
GPL17077 |
| Series (1) |
| GSE49322 |
ALDH1A3-mediated gene expression in MDA-MB-231 cells and MDA-MB-468 cells |
|
