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Sample GSM1197696 Query DataSets for GSM1197696
Status Public on Mar 07, 2014
Title Muc1+_Pdpn+_E18-1
Sample type RNA
 
Source name Muc1+/Pdpn+ cells FACS purified from E18 lungs
Organism Mus musculus
Characteristics tissue: Lung
age: E18
cell population: Muc1+/Pdpn+
Extracted molecule total RNA
Extraction protocol Total RNA was performed using RNeasy Micro Kit from Qiagen according to the manufacturer's instructions.
Label biotin
Label protocol Total RNA was amplified using the Two-Cycle cDNA synthesis kit from Affymetrix following manufacturer's instructions.
Biotinylated aRNA was prepared with the GeneChip 3' IVT Express Kit from Affymetrix following manufacturer's instructions.
 
Hybridization protocol Hybridization of aRNA to Mouse Genome 430 2.0 Array from Affymetrix was achieved following manufacturer's instructions.
Scan protocol GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G.
Description biological rep1
Data processing The data were analyzed with Expression Console using Affymetrix default analysis settings (MAS 5.0).
 
Submission date Jul 30, 2013
Last update date Mar 07, 2014
Contact name Mark Krasnow
Organization name Stanford University
Street address Beckman Center, B400 279 W. Campus Dr.
City Stanford
State/province Ca
ZIP/Postal code 94305
Country USA
 
Platform ID GPL1261
Series (1)
GSE49346 Expression data from adult ATII and E18 Bipotent progenitor cells in the mouse lung

Data table header descriptions
ID_REF
VALUE MAS5 Signal
ABS_CALL indicating whether the transcript was present (P), absent (A), or marginal (M)
DETECTION P-VALUE

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-BioB-5_at 408.534 P 0.0020226
AFFX-BioB-M_at 602.937 P 0.000753643
AFFX-BioB-3_at 466.146 P 5.16732e-05
AFFX-BioC-5_at 1174.85 P 0.000258358
AFFX-BioC-3_at 1808.71 P 4.42873e-05
AFFX-BioDn-5_at 2515.8 P 4.42873e-05
AFFX-BioDn-3_at 3922.27 P 0.000169227
AFFX-CreX-5_at 11921.5 P 5.16732e-05
AFFX-CreX-3_at 12338.9 P 4.42873e-05
AFFX-DapX-5_at 54.1626 P 0.000753643
AFFX-DapX-M_at 1008.51 P 0.000753643
AFFX-DapX-3_at 1409.46 P 7.00668e-05
AFFX-LysX-5_at 24.211 P 0.0309764
AFFX-LysX-M_at 139.045 P 0.00255552
AFFX-LysX-3_at 448.089 P 6.02111e-05
AFFX-PheX-5_at 54.7087 P 0.000856509
AFFX-PheX-M_at 126.222 P 0.00359458
AFFX-PheX-3_at 269.877 P 5.16732e-05
AFFX-ThrX-5_at 13.2737 A 0.5
AFFX-ThrX-M_at 82.7222 P 0.036569

Total number of rows: 45101

Table truncated, full table size 1385 Kbytes.




Supplementary file Size Download File type/resource
GSM1197696_Muc1+_Pdpn+_E18-1.CEL.gz 3.9 Mb (ftp)(http) CEL
GSM1197696_Muc1+_Pdpn+_E18-1.CHP.gz 395.1 Kb (ftp)(http) CHP
Raw data provided as supplementary file
Processed data included within Sample table
Processed data provided as supplementary file

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