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Sample GSM1197697 Query DataSets for GSM1197697
Status Public on Mar 07, 2014
Title Muc1+_Pdpn+_E18-2
Sample type RNA
 
Source name Muc1+/Pdpn+ cells FACS purified from E18 lungs
Organism Mus musculus
Characteristics tissue: Lung
age: E18
cell population: Muc1+/Pdpn+
Extracted molecule total RNA
Extraction protocol Total RNA was performed using RNeasy Micro Kit from Qiagen according to the manufacturer's instructions.
Label biotin
Label protocol Total RNA was amplified using the Two-Cycle cDNA synthesis kit from Affymetrix following manufacturer's instructions.
Biotinylated aRNA was prepared with the GeneChip 3' IVT Express Kit from Affymetrix following manufacturer's instructions.
 
Hybridization protocol Hybridization of aRNA to Mouse Genome 430 2.0 Array from Affymetrix was achieved following manufacturer's instructions.
Scan protocol GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G.
Description biological rep2
Data processing The data were analyzed with Expression Console using Affymetrix default analysis settings (MAS 5.0).
 
Submission date Jul 30, 2013
Last update date Mar 07, 2014
Contact name Mark Krasnow
Organization name Stanford University
Street address Beckman Center, B400 279 W. Campus Dr.
City Stanford
State/province Ca
ZIP/Postal code 94305
Country USA
 
Platform ID GPL1261
Series (1)
GSE49346 Expression data from adult ATII and E18 Bipotent progenitor cells in the mouse lung

Data table header descriptions
ID_REF
VALUE MAS5 Signal
ABS_CALL indicating whether the transcript was present (P), absent (A), or marginal (M)
DETECTION P-VALUE

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-BioB-5_at 459.032 P 0.002867
AFFX-BioB-M_at 687.255 P 0.000753643
AFFX-BioB-3_at 501.561 P 5.16732e-05
AFFX-BioC-5_at 1392.16 P 0.000509415
AFFX-BioC-3_at 2110.58 P 5.16732e-05
AFFX-BioDn-5_at 2822.99 P 4.42873e-05
AFFX-BioDn-3_at 4202.61 P 0.000296708
AFFX-CreX-5_at 11778.7 P 5.16732e-05
AFFX-CreX-3_at 12505.8 P 4.42873e-05
AFFX-DapX-5_at 33.5608 P 0.0044838
AFFX-DapX-M_at 597.019 P 0.000856509
AFFX-DapX-3_at 1194.08 P 9.4506e-05
AFFX-LysX-5_at 16.5527 A 0.175328
AFFX-LysX-M_at 102.664 P 0.0239288
AFFX-LysX-3_at 352.337 P 4.42873e-05
AFFX-PheX-5_at 22.9153 A 0.131361
AFFX-PheX-M_at 71.6228 P 0.00499819
AFFX-PheX-3_at 297.312 P 0.000389797
AFFX-ThrX-5_at 15.8874 A 0.470241
AFFX-ThrX-M_at 43.0388 A 0.0956669

Total number of rows: 45101

Table truncated, full table size 1385 Kbytes.




Supplementary file Size Download File type/resource
GSM1197697_Muc1+_Pdpn+_E18-2.CEL.gz 3.9 Mb (ftp)(http) CEL
GSM1197697_Muc1+_Pdpn+_E18-2.CHP.gz 394.7 Kb (ftp)(http) CHP
Raw data provided as supplementary file
Processed data included within Sample table
Processed data provided as supplementary file

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