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Sample GSM1197698 Query DataSets for GSM1197698
Status Public on Mar 07, 2014
Title Muc1+_Pdpn+_E18-3
Sample type RNA
 
Source name Muc1+/Pdpn+ cells FACS purified from E18 lungs
Organism Mus musculus
Characteristics tissue: Lung
age: E18
cell population: Muc1+/Pdpn+
Extracted molecule total RNA
Extraction protocol Total RNA was performed using RNeasy Micro Kit from Qiagen according to the manufacturer's instructions.
Label biotin
Label protocol Total RNA was amplified using the Two-Cycle cDNA synthesis kit from Affymetrix following manufacturer's instructions.
Biotinylated aRNA was prepared with the GeneChip 3' IVT Express Kit from Affymetrix following manufacturer's instructions.
 
Hybridization protocol Hybridization of aRNA to Mouse Genome 430 2.0 Array from Affymetrix was achieved following manufacturer's instructions.
Scan protocol GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G.
Description biological rep3
Data processing The data were analyzed with Expression Console using Affymetrix default analysis settings (MAS 5.0).
 
Submission date Jul 30, 2013
Last update date Mar 07, 2014
Contact name Mark Krasnow
Organization name Stanford University
Street address Beckman Center, B400 279 W. Campus Dr.
City Stanford
State/province Ca
ZIP/Postal code 94305
Country USA
 
Platform ID GPL1261
Series (1)
GSE49346 Expression data from adult ATII and E18 Bipotent progenitor cells in the mouse lung

Data table header descriptions
ID_REF
VALUE MAS5 Signal
ABS_CALL indicating whether the transcript was present (P), absent (A), or marginal (M)
DETECTION P-VALUE

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-BioB-5_at 568.001 P 0.000509415
AFFX-BioB-M_at 764.004 P 6.02111e-05
AFFX-BioB-3_at 480.579 P 4.42873e-05
AFFX-BioC-5_at 1390.66 P 5.16732e-05
AFFX-BioC-3_at 2253.79 P 4.42873e-05
AFFX-BioDn-5_at 3565.09 P 4.42873e-05
AFFX-BioDn-3_at 5048.42 P 7.00668e-05
AFFX-CreX-5_at 14510.2 P 5.16732e-05
AFFX-CreX-3_at 14777.6 P 4.42873e-05
AFFX-DapX-5_at 8445.92 P 4.42873e-05
AFFX-DapX-M_at 22023.9 P 4.42873e-05
AFFX-DapX-3_at 23631.6 P 4.42873e-05
AFFX-LysX-5_at 4904.3 P 4.42873e-05
AFFX-LysX-M_at 14374.9 P 4.42873e-05
AFFX-LysX-3_at 22423.8 P 4.42873e-05
AFFX-PheX-5_at 8409.96 P 4.42873e-05
AFFX-PheX-M_at 16026.5 P 4.42873e-05
AFFX-PheX-3_at 18747.3 P 4.42873e-05
AFFX-ThrX-5_at 2233.33 P 4.42873e-05
AFFX-ThrX-M_at 13738.4 P 4.42873e-05

Total number of rows: 45101

Table truncated, full table size 1386 Kbytes.




Supplementary file Size Download File type/resource
GSM1197698_Muc1+_Pdpn+_E18-3.CEL.gz 3.8 Mb (ftp)(http) CEL
GSM1197698_Muc1+_Pdpn+_E18-3.CHP.gz 393.8 Kb (ftp)(http) CHP
Raw data provided as supplementary file
Processed data included within Sample table
Processed data provided as supplementary file

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