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Sample GSM1197700 Query DataSets for GSM1197700
Status Public on Mar 07, 2014
Title Lyz2+_EpCAM+_Adult-2
Sample type RNA
 
Source name Lyz2+/EpCAM+ cells isolated from adult lungs
Organism Mus musculus
Characteristics tissue: Lung
age: Adult
cell population: Lyz2+/EpCAM+
Extracted molecule total RNA
Extraction protocol Total RNA was performed using RNeasy Micro Kit from Qiagen according to the manufacturer's instructions.
Label biotin
Label protocol Total RNA was amplified using the Two-Cycle cDNA synthesis kit from Affymetrix following manufacturer's instructions.
Biotinylated aRNA was prepared with the GeneChip 3' IVT Express Kit from Affymetrix following manufacturer's instructions.
 
Hybridization protocol Hybridization of aRNA to Mouse Genome 430 2.0 Array from Affymetrix was achieved following manufacturer's instructions.
Scan protocol GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G.
Description biological rep2
Data processing The data were analyzed with Expression Console using Affymetrix default analysis settings (MAS 5.0).
 
Submission date Jul 30, 2013
Last update date Mar 07, 2014
Contact name Mark Krasnow
Organization name Stanford University
Street address Beckman Center, B400 279 W. Campus Dr.
City Stanford
State/province Ca
ZIP/Postal code 94305
Country USA
 
Platform ID GPL1261
Series (1)
GSE49346 Expression data from adult ATII and E18 Bipotent progenitor cells in the mouse lung

Data table header descriptions
ID_REF
VALUE MAS5 Signal
ABS_CALL indicating whether the transcript was present (P), absent (A), or marginal (M)
DETECTION P-VALUE

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-BioB-5_at 748.975 P 0.000753643
AFFX-BioB-M_at 1172.47 P 0.000753643
AFFX-BioB-3_at 756.648 P 4.42873e-05
AFFX-BioC-5_at 1803.23 P 7.00668e-05
AFFX-BioC-3_at 3399 P 4.42873e-05
AFFX-BioDn-5_at 4245.25 P 4.42873e-05
AFFX-BioDn-3_at 6242.72 P 8.14279e-05
AFFX-CreX-5_at 14312 P 4.42873e-05
AFFX-CreX-3_at 15656.4 P 4.42873e-05
AFFX-DapX-5_at 184.969 P 9.4506e-05
AFFX-DapX-M_at 2833.64 P 0.000581214
AFFX-DapX-3_at 6596.38 P 4.42873e-05
AFFX-LysX-5_at 155.343 P 0.000126798
AFFX-LysX-M_at 782.574 P 4.42873e-05
AFFX-LysX-3_at 2220.21 P 4.42873e-05
AFFX-PheX-5_at 211.171 P 6.02111e-05
AFFX-PheX-M_at 802.056 P 0.000340305
AFFX-PheX-3_at 1445.49 P 5.16732e-05
AFFX-ThrX-5_at 77.8627 P 0.0396608
AFFX-ThrX-M_at 282.06 P 0.0012475

Total number of rows: 45101

Table truncated, full table size 1379 Kbytes.




Supplementary file Size Download File type/resource
GSM1197700_Lyz2+_Epcam+_Adult-2.CEL.gz 3.8 Mb (ftp)(http) CEL
GSM1197700_Lyz2+_Epcam+_Adult-2.CHP.gz 397.6 Kb (ftp)(http) CHP
Raw data provided as supplementary file
Processed data included within Sample table
Processed data provided as supplementary file

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