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Sample GSM1197701 Query DataSets for GSM1197701
Status Public on Mar 07, 2014
Title Lyz2+_EpCAM+_Adult-3
Sample type RNA
 
Source name Lyz2+/EpCAM+ cells isolated from adult lungs
Organism Mus musculus
Characteristics tissue: Lung
age: Adult
cell population: Lyz2+/EpCAM+
Extracted molecule total RNA
Extraction protocol Total RNA was performed using RNeasy Micro Kit from Qiagen according to the manufacturer's instructions.
Label biotin
Label protocol Total RNA was amplified using the Two-Cycle cDNA synthesis kit from Affymetrix following manufacturer's instructions.
Biotinylated aRNA was prepared with the GeneChip 3' IVT Express Kit from Affymetrix following manufacturer's instructions.
 
Hybridization protocol Hybridization of aRNA to Mouse Genome 430 2.0 Array from Affymetrix was achieved following manufacturer's instructions.
Scan protocol GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G.
Description biological rep3
Data processing The data were analyzed with Expression Console using Affymetrix default analysis settings (MAS 5.0).
 
Submission date Jul 30, 2013
Last update date Mar 07, 2014
Contact name Mark Krasnow
Organization name Stanford University
Street address Beckman Center, B400 279 W. Campus Dr.
City Stanford
State/province Ca
ZIP/Postal code 94305
Country USA
 
Platform ID GPL1261
Series (1)
GSE49346 Expression data from adult ATII and E18 Bipotent progenitor cells in the mouse lung

Data table header descriptions
ID_REF
VALUE MAS5 Signal
ABS_CALL indicating whether the transcript was present (P), absent (A), or marginal (M)
DETECTION P-VALUE

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-BioB-5_at 668.641 P 0.000146581
AFFX-BioB-M_at 1050.09 P 4.42873e-05
AFFX-BioB-3_at 591.347 P 5.16732e-05
AFFX-BioC-5_at 1889.82 P 5.16732e-05
AFFX-BioC-3_at 2443.08 P 4.42873e-05
AFFX-BioDn-5_at 4555.47 P 4.42873e-05
AFFX-BioDn-3_at 7082.3 P 7.00668e-05
AFFX-CreX-5_at 17900 P 5.16732e-05
AFFX-CreX-3_at 18149.5 P 4.42873e-05
AFFX-DapX-5_at 14275.9 P 4.42873e-05
AFFX-DapX-M_at 27612.6 P 4.42873e-05
AFFX-DapX-3_at 26269.8 P 4.42873e-05
AFFX-LysX-5_at 10190.4 P 4.42873e-05
AFFX-LysX-M_at 20034 P 4.42873e-05
AFFX-LysX-3_at 29084.5 P 4.42873e-05
AFFX-PheX-5_at 10738.5 P 4.42873e-05
AFFX-PheX-M_at 21269.2 P 4.42873e-05
AFFX-PheX-3_at 24560.5 P 4.42873e-05
AFFX-ThrX-5_at 4319.55 P 4.42873e-05
AFFX-ThrX-M_at 20264.8 P 4.42873e-05

Total number of rows: 45101

Table truncated, full table size 1384 Kbytes.




Supplementary file Size Download File type/resource
GSM1197701_Lyz2+_Epcam+_Adult-3.CEL.gz 3.7 Mb (ftp)(http) CEL
GSM1197701_Lyz2+_Epcam+_Adult-3.CHP.gz 394.5 Kb (ftp)(http) CHP
Raw data provided as supplementary file
Processed data included within Sample table
Processed data provided as supplementary file

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