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Sample GSM119885 Query DataSets for GSM119885
Status Public on Mar 02, 2007
Title hGRB RU 2/23/05 vs. hGRB con 2/23/05 (ExpID 2803)
Sample type RNA
 
Channel 1
Source name hGRB RU 2/23/05 (Cy3)
Organism Homo sapiens
Characteristics hGRB RU 2/23/05
Extracted molecule total RNA
Label Cy3
 
Channel 2
Source name hGRB con 2/23/05 (Cy5)
Organism Homo sapiens
Characteristics hGRB con 2/23/05
Extracted molecule total RNA
Label Cy5
 
 
Description Total RNA was extracted from U-2OSbeta cells treated with either ethanol vehicle or 1uM RU-486 for 6 hours using the RNAqueous Total RNA Isolation Kit. RNA was treated with DNase using the DNA-Free DNase Treatment and Removal Reagents prior to use with the microarray.
Data processing Gene expression analysis was conducted using Agilent Human1Av2 arrays. Total RNA was amplified using the Agilent Low RNA Input Fluorescent Linear Amplification Kit protocol. Starting with 500ng of total RNA, Cy3 or Cy5 labeled cRNA was produced. For each two color comparison, 750ng of each Cy3 and Cy5 labeled cRNAs were mixed and fragmented using the Agilent In Situ Hybridization Kit protocol. Hybridizations were performed for 17 hours in a rotating hybridization oven using the Agilent 60-mer oligo microarray processing protocol. Slides were washed as indicated in this protocol and then scanned with an Agilent Scanner. Data was obtained using the Agilent Feature Extraction software (v7.5), using defaults for all parameters.
 
Submission date Jul 12, 2006
Last update date Jul 06, 2007
Contact name NIEHS Microarray Core
E-mail(s) microarray@niehs.nih.gov, liuliw@niehs.nih.gov
Organization name NIEHS
Department DIR
Lab Microarray Core
Street address 111 T.W. Alexander Drive
City RTP
State/province NC
ZIP/Postal code 27709
Country USA
 
Platform ID GPL887
Series (1)
GSE5310 hGRβ: Elucidation of its Role in Transcriptional Regulation and Identification of a Ligand

Data table header descriptions
ID_REF
rProcessedSignal
gProcessSignal
VALUE Log10(Ratio of Treated:Control)

Data table
ID_REF rProcessedSignal gProcessSignal VALUE
1 488.2867 5512.175 1.0526481019673
2 23.04595 18.85184 -0.08724087017
3 137.7803 129.9519 -0.0254044924273177
4 16322.41 15169.9 -0.0318015647039988
5 3045.229 3262.87 0.0299798139063389
6 19.42154 42.35079 0.338577852327444
7 166.4007 5299.006 1.50303926235014
8 3618.415 3420.371 -0.0244451595294487
9 1164.508 1179.111 0.00541221452546414
10 92.50535 69.12241 -0.126547978958151
11 393.2381 396.6848 0.00378997061727872
12 117.0875 107.0878 -0.0387705366777631
13 193.5698 164.098 -0.0717343133637877
14 168.0493 5500.915 1.51499822651443
15 9333.553 9560.153 0.0104178448162079
16 603.2602 599.4657 -0.0027403348995355
17 65.73986 54.46711 -0.0816944424530247
18 1413.522 1301.632 -0.0358143552380126
20 19.07987 48.84522 0.408246658687483
21 171.8889 5904.259 1.53591756793887

Total number of rows: 22153

Table truncated, full table size 925 Kbytes.




Supplementary file Size Download File type/resource
GSM119885.tif.gz 25.0 Mb (ftp)(http) TIFF
GSM119885.txt.gz 6.2 Mb (ftp)(http) TXT

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