|
| Status |
Public on Jul 28, 2014 |
| Title |
SG13X-C4 |
| Sample type |
RNA |
| |
|
| Source name |
Mus musculus
|
| Organism |
Mus musculus |
| Characteristics |
tissue: xenograft tumor from knockout mice form FLCN-deficient mouse cells
genotype: FLCN deficient
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
R-Phycoerythrin Streptavidin (SAPE), from Molecular Probes, 1 mg/ml ( we use 6 ul per reaction )
|
| Label protocol |
10 ug of Qiagen-purified total RNA
|
| |
|
| Hybridization protocol |
Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome U133 Plus 2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 7G.
|
| Description |
Gene expression ratios were obtained by comparing tumor and normal kidney tissues.
|
| Data processing |
The data were analyzed with custom CDF environments created by the MBNI - CDF: Mm_ENTREZG_11.0.1 was used.
|
| |
|
| Submission date |
Jul 31, 2013 |
| Last update date |
Jul 28, 2014 |
| Contact name |
Karl Dykema |
| Organization name |
Van Andel Institute
|
| Lab |
Lab of Computational Biology
|
| Street address |
333 Bostwick Ave. NE
|
| City |
Grand Rapids |
| State/province |
MI |
| ZIP/Postal code |
49503 |
| Country |
USA |
| |
|
| Platform ID |
GPL8492 |
| Series (1) |
| GSE49397 |
Flcn and BHD renal tumorigenesis |
|
