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Sample GSM119901 Query DataSets for GSM119901
Status Public on Mar 02, 2007
Title hGRB con 2/16/05 vs. U-off con 2/8/05 (ExpID 2833)
Sample type RNA
 
Channel 1
Source name hGRB con 2/16/05 (Cy3)
Organism Homo sapiens
Characteristics hGRB con 2/16/05
Extracted molecule total RNA
Label Cy3
 
Channel 2
Source name U-off con 2/8/05 (Cy5)
Organism Homo sapiens
Characteristics U-off con 2/8/05
Extracted molecule total RNA
Label Cy5
 
 
Description Total RNA was extracted from U-OFF or U-2OSbeta cells treated with ethanol vehicle for 6 hours using the RNAqueous Total RNA Isolation Kit. RNA was treated with DNase using the DNA-Free DNase Treatment and Removal Reagents prior to use with the microarray.
Data processing Gene expression analysis was conducted using Agilent Human1Av2 arrays. Total RNA was amplified using the Agilent Low RNA Input Fluorescent Linear Amplification Kit protocol. Starting with 500ng of total RNA, Cy3 or Cy5 labeled cRNA was produced. For each two color comparison, 750ng of each Cy3 and Cy5 labeled cRNAs were mixed and fragmented using the Agilent In Situ Hybridization Kit protocol. Hybridizations were performed for 17 hours in a rotating hybridization oven using the Agilent 60-mer oligo microarray processing protocol. Slides were washed as indicated in this protocol and then scanned with an Agilent Scanner. Data was obtained using the Agilent Feature Extraction software (v7.5), using defaults for all parameters.
 
Submission date Jul 12, 2006
Last update date Jul 06, 2007
Contact name NIEHS Microarray Core
E-mail(s) microarray@niehs.nih.gov, liuliw@niehs.nih.gov
Organization name NIEHS
Department DIR
Lab Microarray Core
Street address 111 T.W. Alexander Drive
City RTP
State/province NC
ZIP/Postal code 27709
Country USA
 
Platform ID GPL887
Series (1)
GSE5310 hGRβ: Elucidation of its Role in Transcriptional Regulation and Identification of a Ligand

Data table header descriptions
ID_REF
rProcessedSignal
gProcessSignal
VALUE Log10(Ratio of hGRB:U-off)

Data table
ID_REF rProcessedSignal gProcessSignal VALUE
1 371.5786 7110.175 1.28182959588309
2 32.49604 25.83109 -0.0996877080658571
3 92.91443 94.4215 0.00698772846138423
4 19876.35 22104.17 0.0461375764433282
5 3040.788 2785.661 -0.0380578787274879
6 28.32381 29.2078 0.013347173708973
7 67.84614 7310.564 2.03242573928139
8 3762.232 2743.568 -0.137129844276204
9 809.2652 463.554 -0.241990532325958
10 52.17611 28.18393 -0.267470145537926
11 332.9059 388.1688 0.0666991328857251
12 115.3238 99.9031 -0.0623399796369314
13 73.95566 65.4378 -0.0531427271262399
14 62.96862 7769.528 2.09127046059855
15 6230.196 6021.505 -0.0147966583325715
16 345.1329 291.785 -0.0729233986014065
17 29.94872 58.11148 0.287883670821171
18 923.9932 819.9337 -0.051890038435433
20 27.32879 26.67415 -0.0105298143828795
21 90.83092 8281.168 1.95985788220752

Total number of rows: 22153

Table truncated, full table size 919 Kbytes.




Supplementary file Size Download File type/resource
GSM119901.tif.gz 24.6 Mb (ftp)(http) TIFF
GSM119901.txt.gz 6.2 Mb (ftp)(http) TXT

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