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Status |
Public on May 19, 2014 |
Title |
CD138low RPMI-8226 rep 1 |
Sample type |
genomic |
|
|
Source name |
RPMI-8226 cell line
|
Organism |
Homo sapiens |
Characteristics |
cell line: RPMI-8226 subpopulation: CD138low disease: multiple myeloma
|
Treatment protocol |
not applicable
|
Growth protocol |
cells were cultured in RPMI-1640 medium supplemented with 2 mM L-glutamine, 100 U/mL penicillin, 100 mg/mL streptomycin and 10% fetal bovine serum at 37°C and 5% CO2/95% air
|
Extracted molecule |
genomic DNA |
Extraction protocol |
DNA was isolated from CD138++ (n=3) and CD138low (n=3) RPMI-8226 cells using a GenElute Mammalian Genomic DNA Miniprep Kit (Sigma-Aldrich) following the manufacturer’s protocol
|
Label |
biotin
|
Label protocol |
As per manufacturer (Affymetrix)
|
|
|
Hybridization protocol |
DNA was restriction digested, PCR amplified, fragmented, labeled and hybridized to each array according to the manufacturer's instructions (Affymetrix)
|
Scan protocol |
The arrays were processed using the Fluidics Station 450, GeneChip Scanner 3000 7 G and AGCC (AffymetrixGeneChip Command Console Software)
|
Data processing |
.CEL files were generated from the scanned array image files by the Affymetrix GeneChip Command Console Software and were imported into the Affymetrix Chromosome Analysis Suite v1.2.2 Software. Copy number data files (.CYCHP files) were generated using the ChAS Analysis Files for the CytoScan HD Array version NA32.1 as a reference
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|
|
Submission date |
Aug 01, 2013 |
Last update date |
May 19, 2014 |
Contact name |
Teresa Paíno |
E-mail(s) |
tpaino@usal.es
|
Phone |
+34 923294812
|
Organization name |
Centro de investigación del cáncer
|
Street address |
Campus Miguel de Unamuno
|
City |
Salamanca |
ZIP/Postal code |
37007 |
Country |
Spain |
|
|
Platform ID |
GPL16131 |
Series (1) |
GSE49483 |
Phenotypic, genomic and functional characterization reveals no differences between CD138++ and CD138low subpopulations in multiple myeloma cell lines (Copy Number) |
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