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Sample GSM120068 Query DataSets for GSM120068
Status Public on Jul 14, 2006
Title AVC_mutant_rep1
Sample type RNA
 
Source name Atrioventricular canal
Organism Mus musculus
Characteristics Strain: a mixed C57BL6/129 genetic background
Extracted molecule total RNA
Label Ovation Biotin system
Label protocol Ovation isothermal amplification protocol (NuGen)
 
Description Cardiac malformations due to aberrant development of the atrioventricular (AV) valves are among the most common forms of congenital heart disease. At localized swellings of extracellular matrix known as the endocardial cushions, the endothelial lining of the heart undergoes an epithelial to mesenchymal transition (EMT) to form mesenchymal progenitors of the AV valves. Further growth and differentiation of these mesenchymal precursors results in formation of portions of the atrial and ventricular septae, and generation of thin, pliable valves. The transcription factor Gata4 is expressed in the endothelium and mesenchyme of the AV valves. Using a Tie2-Cre transgene, we selectively inactivated Gata4 within endothelial-derived cells. Mutant endothelium failed to undergo EMT, resulting in hypocellular cushions. Mutant cushions had decreased levels of Erbb3, an EGF-family receptor essential for EMT in the atrioventricular cushions. In Gata4 mutant embryos, Erbb3 downregulation was associated with impaired activation of Erk, which is also required for EMT. Expression of a Gata4 mutant protein defective in interaction with Friend of Gata (FOG) cofactors rescued the EMT defect, but resulted in decreased proliferation of mesenchyme and hypoplastic cushions that failed to septate the ventricular inlet. We demonstrate two novel functions of Gata4 in development of the AV valves. First, Gata4 functions as an upstream regulator of an Erbb3-Erk pathway necessary for EMT, and second, Gata4 acts to promote cushion mesenchyme growth and remodeling.
Data processing MAS 5.0, scaled to median intensity array
 
Submission date Jul 12, 2006
Last update date Aug 28, 2018
Contact name Sek Won Kong
E-mail(s) swkong@enders.tch.harvard.edu
Phone 617-919-2689
Organization name Boston Children's Hospital
Department Informatics Program
Lab EN137
Street address 300 Longwood Avenue
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
 
Platform ID GPL1261
Series (1)
GSE5298 Development of heart valves requires Gata4 expression in endothelial-derived cells
Relations
Reanalyzed by GSE119085

Data table header descriptions
ID_REF
VALUE 'Signal' values using MAS 5 algorithm.
ABS_CALL The call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)

Data table
ID_REF VALUE ABS_CALL
1415670_at 822.3338798 P
1415671_at 592.9652238 P
1415672_at 2441.762863 P
1415673_at 497.3317768 P
1415674_a_at 582.2235904 P
1415675_at 1105.830716 P
1415676_a_at 2398.379118 P
1415677_at 347.3208868 P
1415678_at 9106.403604 P
1415679_at 1340.606542 P
1415680_at 386.5907313 P
1415681_at 584.5292417 P
1415682_at 793.1515863 P
1415683_at 1038.887107 P
1415684_at 431.7749946 P
1415685_at 945.5804247 P
1415686_at 7650.350592 P
1415687_a_at 1264.274583 P
1415688_at 4182.835966 P
1415689_s_at 345.878435 P

Total number of rows: 45101

Table truncated, full table size 1114 Kbytes.




Supplementary file Size Download File type/resource
GSM120068.CEL.gz 6.3 Mb (ftp)(http) CEL
Raw data provided as supplementary file

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