|
| Status |
Public on Sep 15, 2013 |
| Title |
miR294_OSK_Day3_Rep2 |
| Sample type |
RNA |
| |
|
| Source name |
OSK-infected MEFs
|
| Organism |
Mus musculus |
| Characteristics |
transgene: Oct4-GFP
treatment: miR181
cell type: OSK-infected MEFs
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Trizol reagent in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
|
| Label |
biotin
|
| Label protocol |
standard Illumina protocol
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
replicate 2
Sample 9
|
| Data processing |
The data were normalised using quantile normalisation with Beadarray in R, and significance in changes between sets determined using Limma
|
| |
|
| Submission date |
Aug 05, 2013 |
| Last update date |
Sep 15, 2013 |
| Contact name |
Robert Blelloch |
| E-mail(s) |
robert.blelloch@ucsf.edu
|
| Phone |
415-476-2839
|
| Organization name |
UCSF
|
| Department |
Urology
|
| Lab |
Blelloch
|
| Street address |
RMB1018, 35 Medical Center Way
|
| City |
San Francisco |
| State/province |
CA |
| ZIP/Postal code |
94143 |
| Country |
USA |
| |
|
| Platform ID |
GPL6885 |
| Series (1) |
| GSE49556 |
The effect of miR-294 and miR-181 on the transcriptome during OSK reprogramming of MEFs |
|
