|
Status |
Public on Aug 01, 2014 |
Title |
Semen_01 |
Sample type |
RNA |
|
|
Source name |
Semen_01
|
Organism |
Homo sapiens |
Characteristics |
tissue: Semen
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA including miRNA was isolated from each body fluids by Qiagen Rneasy Mini Kit according to the manufacturer's instructions.
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol
|
|
|
Hybridization protocol |
Following ligation, ligated miRNA were hybridized for 18 hr at 48℃ on miRNA 3.0 array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
Scan protocol |
miRNA 3.0 array were scanned using the Affymetrix GeneChip scanner 3000
|
Description |
miRNA expression data from semen
|
Data processing |
The data were analyzed with Robust Multi-array average (RMA) using Affymetrix expression console 1.3
|
|
|
Submission date |
Aug 07, 2013 |
Last update date |
Aug 01, 2014 |
Contact name |
Jonglyul Park |
E-mail(s) |
nlcguard@kribb.re.kr
|
Organization name |
Korea Research Institute of Bioscience and Biotechnology
|
Department |
Medical genomics
|
Street address |
52, Eoeun-dong, Yuseong-gu
|
City |
daejeon |
ZIP/Postal code |
305-806 |
Country |
South Korea |
|
|
Platform ID |
GPL16384 |
Series (1) |
GSE49630 |
miRNA expression data from saliva, blood, semen and vaginal swap |
|