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Sample GSM1202833 Query DataSets for GSM1202833
Status Public on Aug 01, 2014
Title Semen_01
Sample type RNA
 
Source name Semen_01
Organism Homo sapiens
Characteristics tissue: Semen
Extracted molecule total RNA
Extraction protocol Total RNA including miRNA was isolated from each body fluids by Qiagen Rneasy Mini Kit according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol
 
Hybridization protocol Following ligation, ligated miRNA were hybridized for 18 hr at 48℃ on miRNA 3.0 array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol miRNA 3.0 array were scanned using the Affymetrix GeneChip scanner 3000
Description miRNA expression data from semen
Data processing The data were analyzed with Robust Multi-array average (RMA) using Affymetrix expression console 1.3
 
Submission date Aug 07, 2013
Last update date Aug 01, 2014
Contact name Jonglyul Park
E-mail(s) nlcguard@kribb.re.kr
Organization name Korea Research Institute of Bioscience and Biotechnology
Department Medical genomics
Street address 52, Eoeun-dong, Yuseong-gu
City daejeon
ZIP/Postal code 305-806
Country South Korea
 
Platform ID GPL16384
Series (1)
GSE49630 miRNA expression data from saliva, blood, semen and vaginal swap

Data table header descriptions
ID_REF
VALUE log2 RMA signal

Data table
ID_REF VALUE
14q0_st 1.356132
14qI-1_st 3.728223
14qI-1_x_st 2.182537
14qI-2_st 1.356132
14qI-3_x_st 0.9308624
14qI-4_st 0.9314564
14qI-4_x_st 1.130679
14qI-5_st 0.6979648
14qI-6_st 0.7632518
14qI-6_x_st 0.6807783
14qI-7_st 0.7388304
14qI-8_st 0.5194296
14qI-8_x_st 0.9033718
14qI-9_x_st 0.6438537
14qII-1_st 1.730585
14qII-1_x_st 1.229914
14qII-10_st 0.6625739
14qII-10_x_st 0.929243
14qII-11_st 2.968794
14qII-11_x_st 1.043039

Total number of rows: 5683

Table truncated, full table size 152 Kbytes.




Supplementary file Size Download File type/resource
GSM1202833_Semen_01.CEL.gz 706.6 Kb (ftp)(http) CEL
Processed data included within Sample table

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