|
| Status |
Public on Aug 01, 2014 |
| Title |
Semen_05 |
| Sample type |
RNA |
| |
|
| Source name |
Semen_05
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: Semen
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA including miRNA was isolated from each body fluids by Qiagen Rneasy Mini Kit according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol
|
| |
|
| Hybridization protocol |
Following ligation, ligated miRNA were hybridized for 18 hr at 48℃ on miRNA 3.0 array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
miRNA 3.0 array were scanned using the Affymetrix GeneChip scanner 3000
|
| Description |
miRNA expression data from semen
|
| Data processing |
The data were analyzed with Robust Multi-array average (RMA) using Affymetrix expression console 1.3
|
| |
|
| Submission date |
Aug 07, 2013 |
| Last update date |
Aug 01, 2014 |
| Contact name |
Jonglyul Park |
| E-mail(s) |
nlcguard@kribb.re.kr
|
| Organization name |
Korea Research Institute of Bioscience and Biotechnology
|
| Department |
Medical genomics
|
| Street address |
52, Eoeun-dong, Yuseong-gu
|
| City |
daejeon |
| ZIP/Postal code |
305-806 |
| Country |
South Korea |
| |
|
| Platform ID |
GPL16384 |
| Series (1) |
| GSE49630 |
miRNA expression data from saliva, blood, semen and vaginal swap |
|
