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Sample GSM1204915 Query DataSets for GSM1204915
Status Public on Aug 10, 2013
Title Res_02
Sample type RNA
 
Source name 10 pooled Dstncorn1 rescued cornea
Organism Mus musculus
Characteristics strain background: mixed; A.BY, C57BL6/J, 129, Swiss Black, and FVB
genotype/variation: Dstncorn1 rescued
age: P58
dox treated: P28-P58
tissue: cornea
Treatment protocol All mice were treated with Doxycycline (ip injection) from P28-P58.
Growth protocol All mice were aged to P58
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol cRNA was synthesized using the Ambion WT Expression kit (Ambion). ss cDNA was synthesized and labeled using the Affymetrix WT Terminal Labeling kit.
 
Hybridization protocol 1 µl (~114 ng) of purified, fragmented, labeled ss cDNA was hybridized to an array at 45°C for 16 h in an Affymetrix 640 hybridization oven.
Scan protocol The post-hybridization process was performed in an Affymetrix 450 fluidic station according to the manufacturer’s instructions. All gene chips were scanned on an Affymetrix GC3000 G7 scanner
Description Rescued 2
gene expression data from 10 pooled Dstncorn1 rescued cornea
Data processing Data were extracted from scanned images using Affymetrix Command Console (v.1.2.0.20). affymetrix-algorithm-name = rma-gene-full (v.1.0)
 
Submission date Aug 08, 2013
Last update date Aug 10, 2013
Contact name Sakae Ikeda
E-mail(s) ikeda@wisc.edu
Organization name University of Wisconsin - Madison
Department Medical Genetics
Lab Ikeda Lab, Room 5350
Street address 425G Henry Mall
City Madison
State/province WI
ZIP/Postal code 53703
Country USA
 
Platform ID GPL16570
Series (1)
GSE49688 Gene expression data from Dstncorn1 mutant, rescued and WT cornea after genetic ablation of Srf from the corneal epithelium

Data table header descriptions
ID_REF
VALUE RMA-normalized

Data table
ID_REF VALUE
17200001 8.03019
17200003 7.64273
17200005 6.34736
17200007 5.9082
17200009 7.66916
17200011 6.80734
17200013 7.27952
17200015 6.97799
17200017 4.80981
17200019 6.11988
17200021 6.20559
17200023 7.97144
17200025 7.44365
17200027 7.64384
17200029 6.68382
17200031 6.0112
17200033 5.71978
17200035 6.37618
17200037 5.97636
17200039 4.69013

Total number of rows: 41345

Table truncated, full table size 683 Kbytes.




Supplementary file Size Download File type/resource
GSM1204915_Res_02_11202012_MoGene-2_0-st_.CEL.gz 9.7 Mb (ftp)(http) CEL
GSM1204915_Res_02_11202012_MoGene-2_0-st_.rma-gene-full.chp.gz 272.9 Kb (ftp)(http) CHP
Processed data included within Sample table
Processed data provided as supplementary file

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