|
| Status |
Public on Aug 10, 2013 |
| Title |
WT_Cont_01_2 |
| Sample type |
RNA |
| |
|
| Source name |
10 pooled WT cornea
|
| Organism |
Mus musculus |
| Characteristics |
strain background: mixed; A.BY, C57BL6/J, 129, Swiss Black, and FVB
genotype/variation: wild type
age: P58
dox treated: P28-P58
tissue: cornea
|
| Treatment protocol |
All mice were treated with Doxycycline (ip injection) from P28-P58.
|
| Growth protocol |
All mice were aged to P58
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
cRNA was synthesized using the Ambion WT Expression kit (Ambion). ss cDNA was synthesized and labeled using the Affymetrix WT Terminal Labeling kit.
|
| |
|
| Hybridization protocol |
1 µl (~114 ng) of purified, fragmented, labeled ss cDNA was hybridized to an array at 45°C for 16 h in an Affymetrix 640 hybridization oven.
|
| Scan protocol |
The post-hybridization process was performed in an Affymetrix 450 fluidic station according to the manufacturer’s instructions. All gene chips were scanned on an Affymetrix GC3000 G7 scanner
|
| Description |
WT control 1
gene expression data from 10 pooled WT cornea
|
| Data processing |
Data were extracted from scanned images using Affymetrix Command Console (v.1.2.0.20). affymetrix-algorithm-name = rma-gene-full (v.1.0)
|
| |
|
| Submission date |
Aug 08, 2013 |
| Last update date |
Aug 10, 2013 |
| Contact name |
Sakae Ikeda |
| E-mail(s) |
ikeda@wisc.edu
|
| Organization name |
University of Wisconsin - Madison
|
| Department |
Medical Genetics
|
| Lab |
Ikeda Lab, Room 5350
|
| Street address |
425G Henry Mall
|
| City |
Madison |
| State/province |
WI |
| ZIP/Postal code |
53703 |
| Country |
USA |
| |
|
| Platform ID |
GPL16570 |
| Series (1) |
| GSE49688 |
Gene expression data from Dstncorn1 mutant, rescued and WT cornea after genetic ablation of Srf from the corneal epithelium |
|
