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Sample GSM1204991 Query DataSets for GSM1204991
Status Public on Jul 15, 2015
Title lung_Cox4 rep2
Sample type RNA
 
Source name lung
Organism Mus musculus
Characteristics Sex: female
strain: C57BL/6N
genotype/variation: Cox4-2 mutant line
age: 17 weeks
mouse id: 30126357
treatment: none
Treatment protocol Mice were sensitized by i.p. injection of 10 µg OVA in 2 mg alum in 200 µg PBS. Mice were subsequently challenged three time by inhalative exposure to OVA aerosol (1% in PBS) for 30 min for weeks after i.p. injection as described in Alessandrini et al, 2006, J Allergy Clin Immunol.
Extracted molecule total RNA
Extraction protocol RNA was extracted with Trizol reagent, followed by clean-up with QIAGEN RNeasy midi kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with gel electrophoreses.
Label biotin
Label protocol Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
 
Hybridization protocol Standard Illumina hybridization protocol
Scan protocol Standard Illumina scanning protocol using HiScan
Description replicate 2
Data processing The data were normalised using cubic spline normalisation with Illumina Genomestudio 2011.1
 
Submission date Aug 09, 2013
Last update date Jul 15, 2015
Contact name Martin Irmler
Organization name Helmholtz Zentrum München GmbH
Department Institute of Experimental Genetics
Lab Gene Regulation & Epigenetics
Street address Ingolstaedter Landstrasse 1
City Neuherberg
State/province Bayern
ZIP/Postal code 85764
Country Germany
 
Platform ID GPL6885
Series (1)
GSE49694 Transcriptome analysis of Cox4-2 knockout mice after OVA challenge

Data table header descriptions
ID_REF
VALUE cubic spline normalized
Detection Pval

Data table
ID_REF VALUE Detection Pval
ILMN_2896528 2487.14 0
ILMN_2721178 521.064 0
ILMN_3033922 761.8984 0
ILMN_3092673 4234.634 0
ILMN_2816356 -0.9871993 0.4536341
ILMN_2808939 442.9068 0
ILMN_2634564 199.0834 0
ILMN_2737647 -1.008406 0.4536341
ILMN_2734484 422.0851 0
ILMN_2952292 48.07637 0.006265664
ILMN_2699078 -7.157307 0.6741855
ILMN_1213681 338.282 0
ILMN_2735413 -8.661479 0.7192982
ILMN_2735415 53.80253 0.005012531
ILMN_2891688 552.4411 0
ILMN_2637698 1689.64 0
ILMN_2674228 204.4249 0
ILMN_2601546 2.337777 0.3533835
ILMN_1230831 -1.138165 0.4561403
ILMN_2848071 8.561524 0.197995

Total number of rows: 25697

Table truncated, full table size 735 Kbytes.




Supplementary data files not provided
Processed data included within Sample table

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