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Sample GSM1205186 Query DataSets for GSM1205186
Status Public on Jul 15, 2015
Title lung wt chall_rep1
Sample type RNA
 
Source name lung
Organism Mus musculus
Characteristics Sex: female
strain: C57BL/6N
genotype/variation: wild type
age: 35 weeks
mouse id: 30144795
treatment: challenged three time by inhalative exposure to OVA aerosol (1% in PBS) for 30 min
Treatment protocol Mice were sensitized by i.p. injection of 10 µg OVA in 2 mg alum in 200 µg PBS. Mice were subsequently challenged three time by inhalative exposure to OVA aerosol (1% in PBS) for 30 min for weeks after i.p. injection as described in Alessandrini et al, 2006, J Allergy Clin Immunol.
Extracted molecule total RNA
Extraction protocol RNA was extracted with Trizol reagent, followed by clean-up with QIAGEN RNeasy midi kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with gel electrophoreses.
Label biotin
Label protocol Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
 
Hybridization protocol Standard Illumina hybridization protocol
Scan protocol Standard Illumina scanning protocol using HiScan
Description replicate 1
Data processing The data were normalised using cubic spline normalisation with Illumina Genomestudio 2011.1
 
Submission date Aug 09, 2013
Last update date Jul 15, 2015
Contact name Martin Irmler
Organization name Helmholtz Zentrum München GmbH
Department Institute of Experimental Genetics
Lab Gene Regulation & Epigenetics
Street address Ingolstaedter Landstrasse 1
City Neuherberg
State/province Bayern
ZIP/Postal code 85764
Country Germany
 
Platform ID GPL6885
Series (1)
GSE49705 Transcriptome analysis of Prdm11 knockout mice after OVA challenge

Data table header descriptions
ID_REF
VALUE cubic spline normalized

Data table
ID_REF VALUE
ILMN_2918977 76.7
ILMN_2918981 80.5
ILMN_1236993 76.7
ILMN_2618329 145.7
ILMN_2939951 319.1
ILMN_1227882 108.9
ILMN_2793827 2247.3
ILMN_2745741 33.2
ILMN_2711892 27.9
ILMN_2676940 103.9
ILMN_1242531 58.6
ILMN_3161404 73.9
ILMN_1225633 83.5
ILMN_2756696 119.2
ILMN_2914510 68.4
ILMN_1257919 35.9
ILMN_1218471 70.6
ILMN_2604393 95.7
ILMN_2828265 62.6
ILMN_2663914 87.7

Total number of rows: 25697

Table truncated, full table size 457 Kbytes.




Supplementary data files not provided
Raw data not provided for this record
Processed data included within Sample table

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