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Sample GSM1207263 Query DataSets for GSM1207263
Status Public on May 21, 2014
Title C57 wild type dexamethasone treatment, biological rep1
Sample type RNA
 
Source name Neurospheres prepared from embryonic day 14.5 mouse cerebral cortex
Organism Mus musculus
Characteristics tissue: embryonic day E14.5 cerebral cortex
genotype: C57 Bl/6 wild type
age: embryonic day E14.5
treatment: 100 nM dexamethasone
Treatment protocol Neurospheres at third passage were treated with 100 nM dexamethasone or equivalent volumes of ethanol vehicle for 4 hours
Growth protocol Cerebral cortex dissected from embryonic day 14.5 fetal mice were dissociated into single cells and then cultured as neurospheres
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label streptavidin phycoerythrin
Label protocol Ambion MessageAmp Premier Enhanced Assay
 
Hybridization protocol Affy Mouse 430A 2.0 Script FS450_0002
Scan protocol standard Affymetrix protocol
Description Gene expression data from wild type C57Bl/6 mice embryonic neural progenitor/stem cells treated with dexamethasone.
Data processing The data is RMA normalized using BRB Array-Tools Software
 
Submission date Aug 12, 2013
Last update date May 21, 2014
Contact name Uma Chandran
E-mail(s) chandran@pitt.edu
Phone 412-648-9326
Organization name University of Pittsburgh
Department BioMedical Informatics
Street address 5150 Center Ave
City Pittsburgh
State/province PA
ZIP/Postal code 15232
Country USA
 
Platform ID GPL8321
Series (1)
GSE49804 Expression data from dexamethasone treated mouse embryonic neural progenitor/stem cells isolated from wild type C57Bl/6 or caveolin-1 knockout mice

Data table header descriptions
ID_REF
VALUE Log2 RMA normalized signal intensities

Data table
ID_REF VALUE
AFFX-BioB-5_at 8.035316
AFFX-BioB-M_at 8.599632
AFFX-BioB-3_at 8.46535
AFFX-BioC-5_at 9.185347
AFFX-BioC-3_at 9.901168
AFFX-BioDn-5_at 10.55112
AFFX-BioDn-3_at 11.62846
AFFX-CreX-5_at 12.40625
AFFX-CreX-3_at 12.71771
AFFX-DapX-5_at 10.20647
AFFX-DapX-M_at 11.05687
AFFX-DapX-3_at 11.64804
AFFX-LysX-5_at 7.874417
AFFX-LysX-M_at 7.922093
AFFX-LysX-3_at 8.958999
AFFX-PheX-5_at 8.406219
AFFX-PheX-M_at 8.444885
AFFX-PheX-3_at 8.834199
AFFX-ThrX-5_at 8.754186
AFFX-ThrX-M_at 9.27066

Total number of rows: 22690

Table truncated, full table size 454 Kbytes.




Supplementary file Size Download File type/resource
GSM1207263_12_0188.CEL.gz 2.0 Mb (ftp)(http) CEL
Processed data included within Sample table

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