|
Status |
Public on May 21, 2014 |
Title |
C57 wild type dexamethasone treatment, biological rep1 |
Sample type |
RNA |
|
|
Source name |
Neurospheres prepared from embryonic day 14.5 mouse cerebral cortex
|
Organism |
Mus musculus |
Characteristics |
tissue: embryonic day E14.5 cerebral cortex genotype: C57 Bl/6 wild type age: embryonic day E14.5 treatment: 100 nM dexamethasone
|
Treatment protocol |
Neurospheres at third passage were treated with 100 nM dexamethasone or equivalent volumes of ethanol vehicle for 4 hours
|
Growth protocol |
Cerebral cortex dissected from embryonic day 14.5 fetal mice were dissociated into single cells and then cultured as neurospheres
|
Extracted molecule |
total RNA |
Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
Label |
streptavidin phycoerythrin
|
Label protocol |
Ambion MessageAmp Premier Enhanced Assay
|
|
|
Hybridization protocol |
Affy Mouse 430A 2.0 Script FS450_0002
|
Scan protocol |
standard Affymetrix protocol
|
Description |
Gene expression data from wild type C57Bl/6 mice embryonic neural progenitor/stem cells treated with dexamethasone.
|
Data processing |
The data is RMA normalized using BRB Array-Tools Software
|
|
|
Submission date |
Aug 12, 2013 |
Last update date |
May 21, 2014 |
Contact name |
Uma Chandran |
E-mail(s) |
chandran@pitt.edu
|
Phone |
412-648-9326
|
Organization name |
University of Pittsburgh
|
Department |
BioMedical Informatics
|
Street address |
5150 Center Ave
|
City |
Pittsburgh |
State/province |
PA |
ZIP/Postal code |
15232 |
Country |
USA |
|
|
Platform ID |
GPL8321 |
Series (1) |
GSE49804 |
Expression data from dexamethasone treated mouse embryonic neural progenitor/stem cells isolated from wild type C57Bl/6 or caveolin-1 knockout mice |
|