|
| Status |
Public on May 21, 2014 |
| Title |
Caveolin-1 knockout dexamethasone treatment, biological rep2 |
| Sample type |
RNA |
| |
|
| Source name |
Neurospheres prepared from embryonic day 14.5 mouse cerebral cortex
|
| Organism |
Mus musculus |
| Characteristics |
tissue: embryonic day E14.5 cerebral cortex
genotype: Caveolin-1 knockout
age: embryonic day E14.5
treatment: 100 nM dexamethasone
|
| Treatment protocol |
Neurospheres at third passage were treated with 100 nM dexamethasone or equivalent volumes of ethanol vehicle for 4 hours
|
| Growth protocol |
Cerebral cortex dissected from embryonic day 14.5 fetal mice were dissociated into single cells and then cultured as neurospheres
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
streptavidin phycoerythrin
|
| Label protocol |
Ambion MessageAmp Premier Enhanced Assay
|
| |
|
| Hybridization protocol |
Affy Mouse 430A 2.0 Script FS450_0002
|
| Scan protocol |
standard Affymetrix protocol
|
| Description |
Gene expression data from caveolin-1 knockout mice embryonic neural progenitor/stem cells treated with dexamethasone.
|
| Data processing |
The data is RMA normalized using BRB Array-Tools Software
|
| |
|
| Submission date |
Aug 12, 2013 |
| Last update date |
May 21, 2014 |
| Contact name |
Uma Chandran |
| E-mail(s) |
chandran@pitt.edu
|
| Phone |
412-648-9326
|
| Organization name |
University of Pittsburgh
|
| Department |
BioMedical Informatics
|
| Street address |
5150 Center Ave
|
| City |
Pittsburgh |
| State/province |
PA |
| ZIP/Postal code |
15232 |
| Country |
USA |
| |
|
| Platform ID |
GPL8321 |
| Series (1) |
| GSE49804 |
Expression data from dexamethasone treated mouse embryonic neural progenitor/stem cells isolated from wild type C57Bl/6 or caveolin-1 knockout mice |
|
