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Sample GSM1207277 Query DataSets for GSM1207277
Status Public on May 21, 2014
Title Caveolin-1 knockout dexamethasone treatment, biological rep4
Sample type RNA
 
Source name Neurospheres prepared from embryonic day 14.5 mouse cerebral cortex
Organism Mus musculus
Characteristics tissue: embryonic day E14.5 cerebral cortex
genotype: Caveolin-1 knockout
age: embryonic day E14.5
treatment: 100 nM dexamethasone
Treatment protocol Neurospheres at third passage were treated with 100 nM dexamethasone or equivalent volumes of ethanol vehicle for 4 hours
Growth protocol Cerebral cortex dissected from embryonic day 14.5 fetal mice were dissociated into single cells and then cultured as neurospheres
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label streptavidin phycoerythrin
Label protocol Ambion MessageAmp Premier Enhanced Assay
 
Hybridization protocol Affy Mouse 430A 2.0 Script FS450_0002
Scan protocol standard Affymetrix protocol
Description Gene expression data from caveolin-1 knockout mice embryonic neural progenitor/stem cells treated with dexamethasone.
Data processing The data is RMA normalized using BRB Array-Tools Software
 
Submission date Aug 12, 2013
Last update date May 21, 2014
Contact name Uma Chandran
E-mail(s) chandran@pitt.edu
Phone 412-648-9326
Organization name University of Pittsburgh
Department BioMedical Informatics
Street address 5150 Center Ave
City Pittsburgh
State/province PA
ZIP/Postal code 15232
Country USA
 
Platform ID GPL8321
Series (1)
GSE49804 Expression data from dexamethasone treated mouse embryonic neural progenitor/stem cells isolated from wild type C57Bl/6 or caveolin-1 knockout mice

Data table header descriptions
ID_REF
VALUE Log2 RMA normalized signal intensities

Data table
ID_REF VALUE
AFFX-BioB-5_at 8.620561
AFFX-BioB-M_at 9.046119
AFFX-BioB-3_at 8.918519
AFFX-BioC-5_at 9.643579
AFFX-BioC-3_at 10.38527
AFFX-BioDn-5_at 11.04572
AFFX-BioDn-3_at 12.13864
AFFX-CreX-5_at 12.97687
AFFX-CreX-3_at 13.34578
AFFX-DapX-5_at 9.573556
AFFX-DapX-M_at 10.46057
AFFX-DapX-3_at 10.92574
AFFX-LysX-5_at 7.084467
AFFX-LysX-M_at 7.362659
AFFX-LysX-3_at 8.326081
AFFX-PheX-5_at 7.862891
AFFX-PheX-M_at 7.993655
AFFX-PheX-3_at 8.609968
AFFX-ThrX-5_at 8.149934
AFFX-ThrX-M_at 8.693211

Total number of rows: 22690

Table truncated, full table size 455 Kbytes.




Supplementary file Size Download File type/resource
GSM1207277_12_0202.CEL.gz 2.1 Mb (ftp)(http) CEL
Processed data included within Sample table

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