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Sample GSM1207280 Query DataSets for GSM1207280
Status Public on May 21, 2014
Title C57 wild type vehicle treatment, biological rep5
Sample type RNA
 
Source name Neurospheres prepared from embryonic day 14.5 mouse cerebral cortex
Organism Mus musculus
Characteristics tissue: embryonic day E14.5 cerebral cortex
genotype: C57 Bl/6 wild type
age: embryonic day E14.5
treatment: ethanol vehicle
Treatment protocol Neurospheres at third passage were treated with 100 nM dexamethasone or equivalent volumes of ethanol vehicle for 4 hours
Growth protocol Cerebral cortex dissected from embryonic day 14.5 fetal mice were dissociated into single cells and then cultured as neurospheres
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label streptavidin phycoerythrin
Label protocol Ambion MessageAmp Premier Enhanced Assay
 
Hybridization protocol Affy Mouse 430A 2.0 Script FS450_0002
Scan protocol standard Affymetrix protocol
Description Gene expression data from wild type C57Bl/6 mice embryonic neural progenitor/stem cells treated with ethanol vehicle.
Data processing The data is RMA normalized using BRB Array-Tools Software
 
Submission date Aug 12, 2013
Last update date May 21, 2014
Contact name Uma Chandran
E-mail(s) chandran@pitt.edu
Phone 412-648-9326
Organization name University of Pittsburgh
Department BioMedical Informatics
Street address 5150 Center Ave
City Pittsburgh
State/province PA
ZIP/Postal code 15232
Country USA
 
Platform ID GPL8321
Series (1)
GSE49804 Expression data from dexamethasone treated mouse embryonic neural progenitor/stem cells isolated from wild type C57Bl/6 or caveolin-1 knockout mice

Data table header descriptions
ID_REF
VALUE Log2 RMA normalized signal intensities

Data table
ID_REF VALUE
AFFX-BioB-5_at 8.624438
AFFX-BioB-M_at 9.012813
AFFX-BioB-3_at 8.865889
AFFX-BioC-5_at 9.601771
AFFX-BioC-3_at 10.33371
AFFX-BioDn-5_at 10.96626
AFFX-BioDn-3_at 12.08492
AFFX-CreX-5_at 12.97184
AFFX-CreX-3_at 13.29023
AFFX-DapX-5_at 9.746605
AFFX-DapX-M_at 10.53125
AFFX-DapX-3_at 11.0511
AFFX-LysX-5_at 7.363075
AFFX-LysX-M_at 7.462712
AFFX-LysX-3_at 8.46744
AFFX-PheX-5_at 8.130003
AFFX-PheX-M_at 8.114944
AFFX-PheX-3_at 8.647336
AFFX-ThrX-5_at 8.362755
AFFX-ThrX-M_at 8.885684

Total number of rows: 22690

Table truncated, full table size 455 Kbytes.




Supplementary file Size Download File type/resource
GSM1207280_12_0205.CEL.gz 2.0 Mb (ftp)(http) CEL
Processed data included within Sample table

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