|
| Status |
Public on Dec 01, 2007 |
| Title |
GFP 3 |
| Sample type |
RNA |
| |
|
| Source name |
gfp-infected
|
| Organism |
Mus musculus |
| Characteristics |
Mouse embryo fibroblast tsc2--
confluent TSC2-/- MEFs
|
| Treatment protocol |
confluent TSC2-/- MEFs were infected with adenovirus expressing GFP for 10 hours, media was changed and cells were harvested 14 hours later.
|
| Growth protocol |
TSC2-/- MEFs were cultured in DMEM containing 10%CCS. Media was changed at time of treatment.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
trizol isolation
|
| Label |
Biotin
|
| Label protocol |
standard affymetrix labelling protocol
|
| |
|
| Hybridization protocol |
standard affymetrix hybridization protocol
|
| Description |
Affymetrix original enzo processing protocol
|
| Data processing |
Data were analyzed by RMA (with default settings) in BioConductor 1.2
Global scaling to TGT 150
|
| |
|
| Submission date |
Jul 18, 2006 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Pere Puigserver |
| E-mail(s) |
Pere_Puigserver@dfci.harvard.edu
|
| Phone |
617-582-7977
|
| Organization name |
Dana-Farber Cancer Institute
|
| Department |
Cancer Biology
|
| Lab |
Smith 936
|
| Street address |
1 Jimmy Fund Way
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02115 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE5332 |
mTOR pathway controls mitochondrial gene expression and respiration through the YY1/PGC-1alpha transcriptional complex |
|
| Relations |
| Reanalyzed by |
GSE119085 |
