|
Status |
Public on Dec 01, 2007 |
Title |
PGC-1alpha 3 |
Sample type |
RNA |
|
|
Source name |
pgc-1alpha-infected
|
Organism |
Mus musculus |
Characteristics |
Mouse embryo fibroblast tsc2-- confluent TSC2-/- MEFs
|
Treatment protocol |
confluent TSC2-/- MEFs were infected with adenovirus expressing PGC-1alpha for 10 hours, media was changed and cells were harvested 14 hours later.
|
Growth protocol |
TSC2-/- MEFs were cultured in DMEM containing 10%CCS. Media was changed at time of treatment.
|
Extracted molecule |
total RNA |
Extraction protocol |
trizol isolation
|
Label |
Biotin
|
Label protocol |
standard affymetrix labelling protocol
|
|
|
Hybridization protocol |
standard affymetrix hybridization protocol
|
Description |
Affymetrix original enzo processing protocol
|
Data processing |
Data were analyzed by RMA (with default settings) in BioConductor 1.2 Global scaling to TGT 150
|
|
|
Submission date |
Jul 18, 2006 |
Last update date |
Aug 28, 2018 |
Contact name |
Pere Puigserver |
E-mail(s) |
Pere_Puigserver@dfci.harvard.edu
|
Phone |
617-582-7977
|
Organization name |
Dana-Farber Cancer Institute
|
Department |
Cancer Biology
|
Lab |
Smith 936
|
Street address |
1 Jimmy Fund Way
|
City |
Boston |
State/province |
MA |
ZIP/Postal code |
02115 |
Country |
USA |
|
|
Platform ID |
GPL1261 |
Series (1) |
GSE5332 |
mTOR pathway controls mitochondrial gene expression and respiration through the YY1/PGC-1alpha transcriptional complex |
|
Relations |
Reanalyzed by |
GSE119085 |