|
| Status |
Public on Sep 13, 2013 |
| Title |
Total mRNA, group C |
| Sample type |
RNA |
| |
|
| Source name |
mouse hypothalamus
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57Bl6
antibody: control
age: adult
genotype: Gck-cre/EF1A1-LSL-EGFPL10
tissue: hypothalamus
|
| Treatment protocol |
Pooled hypothalami from 10 mice were homogenized, centrifuged and mixed with with streptavidin coated Dynal magnetic beads previously incubated with biotinylated protein L and monoclonal mouse anti-GFP antibody. The homogenate represents the total sample. The homogenate and beads were mixed overnight followed by two washes in high potassium wash buffer then a wash in high potassium wash buffer with DTT, cyclohexamide and RNAse inhibitors. The supernatant represents the IP sample.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Agilent Absolutely RNA nanoprep kit. RNA was amplified and converted to cDNA using Ovation PicoSL WTA System (NuGen, San Carlos, CA)
|
| Label |
biotin
|
| Label protocol |
Amplified cDNA was labeled with Encore BiotinIL kit (Nugen)
|
| |
|
| Hybridization protocol |
standard illumina hybridization protocol
|
| Scan protocol |
standard illumina scan protocol
|
| Description |
polysome RNA
|
| Data processing |
data was normalized by quantile normalization using Genespring
|
| |
|
| Submission date |
Aug 14, 2013 |
| Last update date |
Sep 13, 2013 |
| Contact name |
Sarah Stanley |
| E-mail(s) |
sstanley@mail.rockefeller.edu
|
| Organization name |
Rockefeller University
|
| Department |
Dept of Molecular Genetics
|
| Street address |
1230 York Avenue
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10065 |
| Country |
USA |
| |
|
| Platform ID |
GPL6885 |
| Series (1) |
| GSE49868 |
Molecular Profiling of glucokinase-expressing cells from mouse hypothalamus |
|
