|
| Status |
Public on Dec 31, 2014 |
| Title |
Kidney_lupus NZB/W (late remission_Ad-TACI-Ig+CTLA4Ig) rep3 |
| Sample type |
RNA |
| |
|
| Source name |
Kidney from NZB/W mouse under late remission
|
| Organism |
Mus musculus |
| Characteristics |
strain: NZB/W
treatment: Ad-TACI-Ig+CTLA4Ig
disease state: late remission
tissue: Whole kidney
|
| Treatment protocol |
None
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using Trizol and manufacturer's instructions
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from total RNA (Expression Analysis Technical Manual, Affymetrix). GeneChip arrays were scanned by GeneChip Scanner 3000 7G according to the Affymetrix Expression Analysis Technical Manual.
|
| |
|
| Hybridization protocol |
Biotin labeled cDNA was fragmented and hybridized to Affymetrix Mouse Genome 430 2.0 GeneChip arrays (Santa Clara, CA) according to the manufacturer protocol.
|
| Scan protocol |
GeneChip arrays were scanned by GeneChip Scanner 3000 7G according to the Affymetrix Expression Analysis Technical Manual.
|
| Description |
Sample name: Late3_E18LU
Kidney from lupus NZB/W mouse (late remission after Ad-TACI-Ig+CTLA4Ig)
Gene expression data from NZB/W kidney after late remission with Ad-TACI-Ig+CTLA4Ig
|
| Data processing |
The data were analyzed using GenePattern pipeline: RMA method and Mouse Entrez Gene custom CDF annotation version 10 were used for the normalization; and Combat for batch correction within the NZB/W mouse model and the NZM2410 mouse model.
|
| |
|
| Submission date |
Aug 15, 2013 |
| Last update date |
Dec 31, 2014 |
| Contact name |
Celine C Berthier |
| Organization name |
University of Michigan
|
| Street address |
1150 W. Medical Center Dr, MSRB2
|
| City |
Ann Arbor |
| State/province |
MI |
| ZIP/Postal code |
48109 |
| Country |
USA |
| |
|
| Platform ID |
GPL7546 |
| Series (2) |
| GSE49898 |
Identification of stage specific genes associated with lupus nephritis and response to remission induction in NZB/W and NZM2410 mice |
| GSE50038 |
Immunopathogenesis of renal inflammation during the progression, remission and relapse of renal disease in the NZB/W murine lupus model |
|
