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Sample GSM1210744 Query DataSets for GSM1210744
Status Public on Aug 19, 2013
Title HCV-h B4-CD4+ T-cells
Sample type RNA
 
Source name HCV-h CD4+ T-cells
Organism Homo sapiens
Characteristics sample group: long-term treatment-naïve chronic HCV (CHC) patients
hcv viral load: high (HCV-h)
tissue: blood
cell type: CD4+ T-cells
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA from enriched T-cells was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1μg total RNA (GeneChip® Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation and preparation, 10μg of cRNA were hybridized for 16 hr at 45℃ on Affymetrix Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the Affymetrix ® GeneChip® Scanner 3000.
Description Gene expression data from enriched global CD4+ T cells from a chronic HCV infected patients with high viral load
Data processing The data were analyzed with Partek Genomics Suite software (v.6.5) using default analysis settings and normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
 
Submission date Aug 18, 2013
Last update date Nov 30, 2020
Contact name Binbin Zhao
E-mail(s) zhbbzj81@gmail.com
Organization name Institute of Pathogen Biology, CAMS&PUMC
Street address No. 9 Dong Dan San Tiao, Dongcheng District
City Beijing
ZIP/Postal code 100730
Country China
 
Platform ID GPL570
Series (1)
GSE49954 T lymphocytes from Chronic HCV-infected patients express unique pro-apoptotic gene signature.
Relations
Reanalyzed by GSE162329

Data table header descriptions
ID_REF
VALUE log2 normalized

Data table
ID_REF VALUE
1007_s_at 5.36007
1053_at 5.58566
117_at 5.61712
121_at 6.93202
1255_g_at 2.00568
1294_at 6.97296
1316_at 5.4587
1320_at 2.82815
1405_i_at 9.61825
1431_at 3.46743
1438_at 4.1254
1487_at 6.23873
1494_f_at 4.53442
1552256_a_at 5.43476
1552257_a_at 5.69745
1552258_at 5.49401
1552261_at 3.20962
1552263_at 6.6229
1552264_a_at 6.10726
1552266_at 1.81535

Total number of rows: 54675

Table truncated, full table size 998 Kbytes.




Supplementary file Size Download File type/resource
GSM1210744_101209-10758C_B4-3_HG-U133_Plus_2_.CEL.gz 4.4 Mb (ftp)(http) CEL
Processed data included within Sample table

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