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Sample GSM1210767 Query DataSets for GSM1210767
Status Public on Aug 19, 2013
Title HCV-l B39-CD8+ T-cells
Sample type RNA
 
Source name HCV-l CD8+ T-cells
Organism Homo sapiens
Characteristics sample group: long-term treatment-naïve chronic HCV (CHC) patients
hcv viral load: low (HCV-l)
tissue: blood
cell type: CD8+ T-cells
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA from enriched T-cells was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1μg total RNA (GeneChip® Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation and preparation, 10μg of cRNA were hybridized for 16 hr at 45℃ on Affymetrix Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the Affymetrix ® GeneChip® Scanner 3000.
Description Gene expression data from enriched global CD8+ T cells from a chronic HCV infected patients with low viral load
Data processing The data were analyzed with Partek Genomics Suite software (v.6.5) using default analysis settings and normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
 
Submission date Aug 18, 2013
Last update date Nov 30, 2020
Contact name Binbin Zhao
E-mail(s) zhbbzj81@gmail.com
Organization name Institute of Pathogen Biology, CAMS&PUMC
Street address No. 9 Dong Dan San Tiao, Dongcheng District
City Beijing
ZIP/Postal code 100730
Country China
 
Platform ID GPL570
Series (1)
GSE49954 T lymphocytes from Chronic HCV-infected patients express unique pro-apoptotic gene signature.
Relations
Reanalyzed by GSE162329

Data table header descriptions
ID_REF
VALUE log2 normalized

Data table
ID_REF VALUE
1007_s_at 5.54148
1053_at 6.36422
117_at 4.72573
121_at 7.0381
1255_g_at 1.82055
1294_at 7.18884
1316_at 5.85466
1320_at 2.76936
1405_i_at 12.4088
1431_at 3.11423
1438_at 3.88931
1487_at 5.983
1494_f_at 4.43641
1552256_a_at 4.76087
1552257_a_at 5.93699
1552258_at 5.47189
1552261_at 3.10075
1552263_at 7.44882
1552264_a_at 6.9749
1552266_at 1.34534

Total number of rows: 54675

Table truncated, full table size 998 Kbytes.




Supplementary file Size Download File type/resource
GSM1210767_101209-10758C_B39-2_HG-U133_Plus_2_.CEL.gz 4.2 Mb (ftp)(http) CEL
Processed data included within Sample table

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