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Sample GSM1213132 Query DataSets for GSM1213132
Status Public on Aug 26, 2013
Title LSEC sham operated-4
Sample type RNA
 
Source name LSEC
Organism Mus musculus
Characteristics strain: C57Bl/6N
cell type: liver sinusoidal endothelial cells
treatment: sham
age: 8 weeks
Treatment protocol No treatment. LSEC were directly lysed in TRIZOL after isolation.
Growth protocol no in vitro culture. LSEC were directly lysed in TRIZOL after isolation.
Extracted molecule total RNA
Extraction protocol RNA was extracted with Trizol reagent, followed by clean-up with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Then RNA were dissoved in Rnase-free water. Quality control was performed with Agilent Bioanalyser.
Label biotin
Label protocol Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
 
Hybridization protocol Standard Illumina hybridization protocol
Scan protocol Standard Illumina scanning protocol
Data processing The data were normalised using quantile normalisation with IlluminaGUI in R
 
Submission date Aug 20, 2013
Last update date Aug 26, 2013
Contact name Junhao Hu
E-mail(s) j.hu@dkfz.de
Phone +496221421504
Organization name German Cancer Research Center
Department Division of Vascular Oncology and Metastasis
Street address Im Neuenheimer Feld 280
City Heidelberg
ZIP/Postal code 69120
Country Germany
 
Platform ID GPL6885
Series (1)
GSE50046 Whole genome profiling of liver sinusoidal endothelial cells (LSEC) before and after hepatectomy

Data table header descriptions
ID_REF
VALUE quantile normalized
WT_sham4_6085341004_D.sd
WT_sham4_6085341004_D.p
WT_sham4_6085341004_D.nbeads

Data table
ID_REF VALUE WT_sham4_6085341004_D.sd WT_sham4_6085341004_D.p WT_sham4_6085341004_D.nbeads
ILMN_1250052 107.5859534 23.66835431 0.454379511 34
ILMN_3122480 103.8733319 27.16072543 0.754312283 34
ILMN_2599935 232.4410912 66.31912015 3.0452E-14 38
ILMN_2675543 108.1280845 20.87191028 0.38356305 38
ILMN_2686883 112.1238519 18.75622346 0.084806767 28
ILMN_2751818 106.8630931 28.25468809 0.519101421 29
ILMN_2728634 155.6100261 46.61235961 1.35981E-09 49
ILMN_3040515 110.2787048 23.19338662 0.197155889 40
ILMN_2711608 104.4933273 27.5645202 0.743387333 48
ILMN_1232875 158.769462 49.90601417 2.13705E-08 42
ILMN_1258507 101.4724923 21.7829532 0.95522522 45
ILMN_2746142 102.7046223 23.21491947 0.858271291 33
ILMN_2655499 101.8137844 19.72730632 0.953321624 41
ILMN_1252870 118.4345072 33.4933521 0.025184494 36
ILMN_1248179 107.4184481 30.68510092 0.474469176 43
ILMN_2649955 150.1321129 36.80166635 5.85345E-11 49
ILMN_2628708 123.6962734 34.41217887 0.006632119 30
ILMN_3024781 96.74139225 15.97810584 0.999546587 32
ILMN_2705628 194.2953988 55.74971634 1.12883E-14 48
ILMN_2689731 283.1879067 55.18927459 1.30648E-23 42

Total number of rows: 25622

Table truncated, full table size 1292 Kbytes.




Supplementary data files not provided
Processed data included within Sample table
Processed data are available on Series record

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