|
| Status |
Public on Aug 26, 2013 |
| Title |
LSEC hepatectomized-3 |
| Sample type |
RNA |
| |
|
| Source name |
LSEC
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57Bl/6N
cell type: liver sinusoidal endothelial cells
treatment: hepatectomized
age: 8 weeks
|
| Treatment protocol |
No treatment. LSEC were directly lysed in TRIZOL after isolation.
|
| Growth protocol |
no in vitro culture. LSEC were directly lysed in TRIZOL after isolation.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Trizol reagent, followed by clean-up with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Then RNA were dissoved in Rnase-free water. Quality control was performed with Agilent Bioanalyser.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Data processing |
The data were normalised using quantile normalisation with IlluminaGUI in R
|
| |
|
| Submission date |
Aug 20, 2013 |
| Last update date |
Aug 26, 2013 |
| Contact name |
Junhao Hu |
| E-mail(s) |
j.hu@dkfz.de
|
| Phone |
+496221421504
|
| Organization name |
German Cancer Research Center
|
| Department |
Division of Vascular Oncology and Metastasis
|
| Street address |
Im Neuenheimer Feld 280
|
| City |
Heidelberg |
| ZIP/Postal code |
69120 |
| Country |
Germany |
| |
|
| Platform ID |
GPL6885 |
| Series (1) |
| GSE50046 |
Whole genome profiling of liver sinusoidal endothelial cells (LSEC) before and after hepatectomy |
|
