|
| Status |
Public on Aug 23, 2013 |
| Title |
AGM-W433-vehicle treated |
| Sample type |
RNA |
| |
|
| Source name |
AGM-vehicle treated
|
| Organism |
Chlorocebus aethiops |
| Characteristics |
tissue: liver biopsy
treatment: vehicle
|
| Treatment protocol |
Before the administration of the antimiR compounds the animals were randomly divided into 4 groups of 5 animals. The antimiR-33a, antimiR-33b and antimiR-33a/b compounds were subcutaneously delivered at a dose of 20 mg/kg on day 0, and a 5 mg/kg maintenance dose at weekly intervals. Vehicle controls received a 0.9% NaCl solution. At day 57 (after 8 weeks of treatment) liver biopsies were taken for this microarray analysis)
|
| Growth protocol |
Twenty moderately obese adult female African green monkeys were selected for the study and fed a high-fat diet for 12 weeks. All monkeys were housed at the RxGen primate facility on the St. Kitts Biomedical Research Foundation campus, St. Kitts
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Double-stranded cDNA (ds cDNA) was prepared from 1 µg of total RNA using the cDNA Synthesis System (Roche).
|
| Label |
Cy3
|
| Label protocol |
DsDNA was labeled with Cy3 using the Roche One Color DNA Labeling kit following the manufacturer’s protocol.
|
| |
|
| Hybridization protocol |
Six μg of labeled cDNA was hybridized to the array for 20 hours following the manufacturer’s protocol. Arrays were washed using the NimbleGen wash buffer kit following the manufacturer’s protocol.
|
| Scan protocol |
Arrays were scanned on the ms200 scanner. Images were aligned and features were extracted using Nimblescan 2.6 software.
|
| Description |
Female obese African Green Monkey on a high fat diet, treated with vehicle only, RNA from liver biopsy
|
| Data processing |
Data were analyzed with the Agilent Genespring GX 11.0
|
| |
|
| Submission date |
Aug 22, 2013 |
| Last update date |
Aug 23, 2013 |
| Contact name |
Veerle Rottiers |
| Organization name |
Massachusetts General Hospital
|
| Department |
Cancer Center
|
| Lab |
AM Näär
|
| Street address |
149, 13th Street
|
| City |
Charlestown |
| State/province |
MA |
| ZIP/Postal code |
02129 |
| Country |
USA |
| |
|
| Platform ID |
GPL16025 |
| Series (1) |
| GSE50116 |
Expression analysis of African Green Monkeys after treatment with a miR-33a/b-targeting LNA-antimiR |
|
