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Status |
Public on Aug 23, 2013 |
Title |
AGM-Z067-vehicle treated |
Sample type |
RNA |
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|
Source name |
AGM-vehicle treated
|
Organism |
Chlorocebus aethiops |
Characteristics |
tissue: liver biopsy treatment: vehicle
|
Treatment protocol |
Before the administration of the antimiR compounds the animals were randomly divided into 4 groups of 5 animals. The antimiR-33a, antimiR-33b and antimiR-33a/b compounds were subcutaneously delivered at a dose of 20 mg/kg on day 0, and a 5 mg/kg maintenance dose at weekly intervals. Vehicle controls received a 0.9% NaCl solution. At day 57 (after 8 weeks of treatment) liver biopsies were taken for this microarray analysis)
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Growth protocol |
Twenty moderately obese adult female African green monkeys were selected for the study and fed a high-fat diet for 12 weeks. All monkeys were housed at the RxGen primate facility on the St. Kitts Biomedical Research Foundation campus, St. Kitts
|
Extracted molecule |
total RNA |
Extraction protocol |
Double-stranded cDNA (ds cDNA) was prepared from 1 µg of total RNA using the cDNA Synthesis System (Roche).
|
Label |
Cy3
|
Label protocol |
DsDNA was labeled with Cy3 using the Roche One Color DNA Labeling kit following the manufacturer’s protocol.
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|
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Hybridization protocol |
Six μg of labeled cDNA was hybridized to the array for 20 hours following the manufacturer’s protocol. Arrays were washed using the NimbleGen wash buffer kit following the manufacturer’s protocol.
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Scan protocol |
Arrays were scanned on the ms200 scanner. Images were aligned and features were extracted using Nimblescan 2.6 software.
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Description |
Female obese African Green Monkey on a high fat diet, treated with vehicle only, RNA from liver biopsy
|
Data processing |
Data were analyzed with the Agilent Genespring GX 11.0
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|
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Submission date |
Aug 22, 2013 |
Last update date |
Aug 23, 2013 |
Contact name |
Veerle Rottiers |
Organization name |
Massachusetts General Hospital
|
Department |
Cancer Center
|
Lab |
AM Näär
|
Street address |
149, 13th Street
|
City |
Charlestown |
State/province |
MA |
ZIP/Postal code |
02129 |
Country |
USA |
|
|
Platform ID |
GPL16025 |
Series (1) |
GSE50116 |
Expression analysis of African Green Monkeys after treatment with a miR-33a/b-targeting LNA-antimiR |
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