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Sample GSM1219080 Query DataSets for GSM1219080
Status Public on Aug 24, 2014
Title Ethanol-treated A29
Sample type RNA
 
Source name treated mouse
Organism Mus musculus
Characteristics strain: C57BL/6J x FVB/NJ F1
tissue: frontal cortex
stress: ethanol
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using Ambion's mirVana™ miRNA Isolation Kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
Label biotin
Label protocol Biotinylated cRNA were prepared with the Illumina® TotalPrep RNA Amplification Kit
 
Hybridization protocol Standard Illumina hybridization protocol
Scan protocol Standard Illumina scanning protocol
Description 5665159054_C
treated mouse
Data processing The data were normalized using quantile normalization with lumi package in R
The quality of the Illumina bead summary data was assessed using the Bioconductor packages Lumi and arrayQualityMetrics. Data preprocessing included variance stabilization and quantile normalization using the Lumi package. Statistical analysis comparing ethanol-treated and control groups was performed using the Bioconductor package limma, which implements an empirical Bayes approach in R (Smyth 2005). False discovery rate (FDR) was assessed using the Benjamini-Hochberg method.
 
Submission date Aug 29, 2013
Last update date Aug 24, 2014
Contact name Yury O. Nunez
E-mail(s) nunezyo@gmail.com
Phone 210-567-8094
Organization name University of Texas at Austin
Department Pharmacy
Lab Pharmacogenomics
Street address 7703 Floyd Curl Drive MC6220
City San Antonio
State/province TX
ZIP/Postal code 78229
Country USA
 
Platform ID GPL6885
Series (1)
GSE50427 Gene (mRNA) expression profiling in frontal cortex of ethanol-treated mice

Data table header descriptions
ID_REF
VALUE Quantile normalized signal (AVG_Signal)
Detection Pval
BEAD_STDERR
Avg_NBEADS

Data table
ID_REF VALUE Detection Pval BEAD_STDERR Avg_NBEADS
ILMN_2896528 1919.543 0.00 119.6156 47
ILMN_2721178 678.3689 0.00 40.76519 48
ILMN_3033922 1179.471 0.00 75.67142 48
ILMN_3092673 4319.806 0.00 210.2412 39
ILMN_2816356 173.68 0.08897243 8.495487 46
ILMN_2808939 665.7599 0.00 43.43634 32
ILMN_2634564 528.3931 0.00 38.23056 30
ILMN_2737647 181.7255 0.04010025 12.30641 29
ILMN_2734484 436.9901 0.00 35.74265 44
ILMN_2952292 182.5048 0.03759398 7.808636 55
ILMN_2699078 144.3439 0.8245614 6.428351 41
ILMN_1213681 368.8617 0.00 24.09533 43
ILMN_2735413 140.5732 0.8947368 6.444898 35
ILMN_2735415 165.4507 0.1867168 10.88926 27
ILMN_2891688 626.2228 0.00 37.74806 34
ILMN_2637698 1014.485 0.00 88.64176 34
ILMN_2674228 366.2644 0.00 20.82605 42
ILMN_2601546 153.1967 0.5338346 8.633382 47
ILMN_1230831 170.7979 0.1190476 7.52821 35
ILMN_2848071 181.8896 0.04010025 10.7319 32

Total number of rows: 25697

Table truncated, full table size 1053 Kbytes.




Supplementary data files not provided
Raw data are available on Series record
Processed data included within Sample table

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