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Sample GSM1220312 Query DataSets for GSM1220312
Status Public on Sep 23, 2013
Title Knockout (KO) 4 wk CL
Sample type RNA
 
Source name MED1 KO_4wk_CL
Organism Mus musculus
Characteristics strain background: C57BL/6
genotype/variation: MED1 fl/fl K14 driven Cre
age: 4 weeks
tissues: microdissected dental tissues from lower incisors
Stage: Cervical Loop (CL)
Treatment protocol Dental tissues generating hairs were dissected from MED1 KO mouse lower incisors, and the tissues from 3 mice were combined, then RNA extracted. Equivalent tissues in control littermate mice were also dissected.
Growth protocol Coactivator MED1 is removed from keratin 14 (K14) expressing epithelia by using Crelox stragety
Extracted molecule total RNA
Extraction protocol Total RNA was isolated from dissected dental tissues by using Pico pure RNA purification kit (ABI)
Label biotin
Label protocol Standard Illumina hybridization protocol; cDNA is labeled by biotin and hybridized with Cy3-streptoavidin
 
Hybridization protocol Standard Illumina hybridization protocol done by UCLA neuroscience genomics core
Scan protocol Standard Illumina hybridication protocol done by UCLA neuroscience genomics core
Description KO CL
Data processing data were normalized by using quantile method in Genome studio (v.V2011.1; Module Gene Expression v.1.9.0).
 
Submission date Aug 30, 2013
Last update date Sep 23, 2013
Contact name Yuko Oda
E-mail(s) yuko.oda@ucsf.edu
Phone 4152692370
Organization name VA Medical Center UCSF
Department Endocrine Research
Street address 111N-MB 360 1700 Owens St, Suite 360
City San Francisco
State/province CA
ZIP/Postal code 94158
Country USA
 
Platform ID GPL6885
Series (2)
GSE50503 Ablation of coactivator Med1 switches the cell fate of dental epithelia to that generating hair [mixed stage]
GSE50504 Ablation of coactivator Med1 switches the cell fate of dental epithelia to that generating hair

Data table header descriptions
ID_REF
VALUE quantile normalized using Genome Studio
Detection Pval

Data table
ID_REF VALUE Detection Pval
ILMN_1248788 138.5 0.51754
ILMN_2707227 162.4 0.09273
ILMN_2896528 6960.3 0
ILMN_2721178 1993.1 0
ILMN_1227723 787 0
ILMN_3033922 2093.9 0
ILMN_3092673 8316 0
ILMN_2730714 6610.4 0
ILMN_3162224 2248 0
ILMN_2816356 209.6 0.00376
ILMN_2808939 1821 0
ILMN_2634564 952.8 0
ILMN_1216623 1110.8 0
ILMN_1215157 1260.1 0
ILMN_1228777 138.9 0.49749
ILMN_2737647 158.9 0.12531
ILMN_1216425 132.8 0.74561
ILMN_2734484 867.6 0
ILMN_2652961 134 0.69173
ILMN_2952292 214.6 0.00376

Total number of rows: 25697

Table truncated, full table size 613 Kbytes.




Supplementary data files not provided
Raw data are available on Series record
Processed data included within Sample table

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