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Sample GSM1220314 Query DataSets for GSM1220314
Status Public on Sep 23, 2013
Title KO 4 wk Sec
Sample type RNA
 
Source name MED1 KO_4wk_Sec
Organism Mus musculus
Characteristics strain background: C57BL/6
genotype/variation: MED1 fl/fl K14 driven Cre
age: 4 weeks
tissues: microdissected dental tissues from lower incisors
Stage: Secretion Stage (Sec)
Treatment protocol Dental tissues generating hairs were dissected from MED1 KO mouse lower incisors, and the tissues from 3 mice were combined, then RNA extracted. Equivalent tissues in control littermate mice were also dissected.
Growth protocol Coactivator MED1 is removed from keratin 14 (K14) expressing epithelia by using Crelox stragety
Extracted molecule total RNA
Extraction protocol Total RNA was isolated from dissected dental tissues by using Pico pure RNA purification kit (ABI)
Label biotin
Label protocol Standard Illumina hybridization protocol; cDNA is labeled by biotin and hybridized with Cy3-streptoavidin
 
Hybridization protocol Standard Illumina hybridization protocol done by UCLA neuroscience genomics core
Scan protocol Standard Illumina hybridication protocol done by UCLA neuroscience genomics core
Description KO Sec
Data processing data were normalized by using quantile method in Genome studio (v.V2011.1; Module Gene Expression v.1.9.0).
 
Submission date Aug 30, 2013
Last update date Sep 23, 2013
Contact name Yuko Oda
E-mail(s) yuko.oda@ucsf.edu
Phone 4152692370
Organization name VA Medical Center UCSF
Department Endocrine Research
Street address 111N-MB 360 1700 Owens St, Suite 360
City San Francisco
State/province CA
ZIP/Postal code 94158
Country USA
 
Platform ID GPL6885
Series (2)
GSE50503 Ablation of coactivator Med1 switches the cell fate of dental epithelia to that generating hair [mixed stage]
GSE50504 Ablation of coactivator Med1 switches the cell fate of dental epithelia to that generating hair

Data table header descriptions
ID_REF
VALUE quantile normalized using Genome Studio
Detection Pval

Data table
ID_REF VALUE Detection Pval
ILMN_1248788 131.4 0.77068
ILMN_2707227 171.5 0.05388
ILMN_2896528 5190.8 0
ILMN_2721178 1478.7 0
ILMN_1227723 715.9 0
ILMN_3033922 1788.5 0
ILMN_3092673 7935.8 0
ILMN_2730714 8436.4 0
ILMN_3162224 1841.8 0
ILMN_2816356 187.2 0.01128
ILMN_2808939 1177.8 0
ILMN_2634564 493.9 0
ILMN_1216623 607.6 0
ILMN_1215157 649.4 0
ILMN_1228777 140.1 0.44737
ILMN_2737647 145.6 0.29574
ILMN_1216425 140.3 0.4411
ILMN_2734484 985.7 0
ILMN_2652961 122.5 0.98246
ILMN_2952292 208.9 0.00251

Total number of rows: 25697

Table truncated, full table size 612 Kbytes.




Supplementary data files not provided
Raw data are available on Series record
Processed data included within Sample table

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