|
| Status |
Public on Nov 10, 2013 |
| Title |
C2ATF2 replicate1 |
| Sample type |
RNA |
| |
|
| Source name |
Hepatoblasts expressing active ATF2 8hrs
|
| Organism |
Mus musculus |
| Characteristics |
cell type: Embryonic hepatoblasts
|
| Growth protocol |
Hepatoblasts were seeded onto gelatin coated plates. Medium was DMEM (sigma D0819), plus 10% FBS, 1% penicillin/streptomycin, HGF 10ng/ml, EGF 20 ng/ml, dexamethasone 1uM.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using Rneasy columns (QIAgen 74104)
|
| Label |
biotin
|
| Label protocol |
RNA was reverse transcribed using an Ovation Pico WTA kit (NuGen, 3300-12) to produce an amplified cDNA. Second strand synthesis was achieved using a WT-Ovation Exon module version 1.0 kit (NuGen, 2000-12), after which the cDNA was fragmented and labelled using Encore Biotin Module reagents (NuGen, 4200-12).
|
| |
|
| Hybridization protocol |
The products were hybridized to GeneChip Mouse Gene Array 1.0 ST (Affymetrix) microarray chips. Hybridized arrays were washed using a GeneChip Fluidics Station 450 (Affymetrix).
|
| Scan protocol |
Scanned using a GeneChip Scanner 30007G (Affymetrix).
|
| Data processing |
The data were analyzed with RMA implemented in the software apt-probeset-summarize with the default settings.
|
| |
|
| Submission date |
Sep 03, 2013 |
| Last update date |
Nov 10, 2013 |
| Contact name |
steve lyons |
| E-mail(s) |
slyons@picr.man.ac.uk
|
| Phone |
0161 446 3028
|
| Organization name |
CRUK Manchester
|
| Department |
Cell Regulation
|
| Street address |
Wilmslow Rd
|
| City |
Manchester |
| ZIP/Postal code |
M20 4BX |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL10740 |
| Series (1) |
| GSE50530 |
Identification of ATF2 target genes |
|
