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Sample GSM1224728 Query DataSets for GSM1224728
Status Public on Sep 06, 2013
Title activated WT T-cells day 4 rep1
Sample type RNA
 
Source name Balb/c mouse WT1
Organism Mus musculus
Characteristics cell type: CD3+ T-cells
genotype: WT
age: 12 wk
genetic background: Balb/C
gender: Male
Treatment protocol Isolated cells were cultured on tissue culture plates with 3 mg/ml of plate-coated anti-mouse CD3 antibody and 3 mg//ml of soluble anti-mouse CD28 antibody for 4 days.
Growth protocol Isolated CD3+ T-cells were cultured in RPMI supplemented with 10% FBS, 100 U/mL penicillin/streptomycin, and 55 mM 2-mercaptoethanol at 37°C in 95% humidity and 5% CO2.
Extracted molecule total RNA
Extraction protocol Total RNA was isolated using the RNeasy Kit from Qiagen. We followed company's instruction.
Label biotin
Label protocol 100 ng/ml total RNA was reverse transcribed with T7 oligo(dT) primer and amplified. After amplification, multiple copies of biotin-modified aRNA was generated by using Affymetrix GeneChip 3' IVT Express Kit according to manufacturer's protocol.
 
Hybridization protocol All prepared samples were labeled and hybridized to Affymetrix mouse Gene ST 1.0 GeneChip.
Scan protocol Affymetrix GeneChip scanner 3000 was used for scanning and Affymetrix GCOS software was used for data collection.
Description Gene expression data from WT Balb/C T-cells
Data processing RMA
 
Submission date Sep 05, 2013
Last update date Sep 06, 2013
Contact name Seok-Joo Kwon
Organization name NCI/NIH
Street address 37 Convent Dr.
City Bethesda
State/province MD
ZIP/Postal code 20892
Country USA
 
Platform ID GPL10740
Series (1)
GSE50617 Expression data from mouse CD3+ T-cells from WT and KLF13 KO mice

Data table header descriptions
ID_REF
VALUE log2 RMA signal
DETECTION P-VALUE

Data table
ID_REF VALUE DETECTION P-VALUE
10338001 13.0412 0
10338002 6.2326 0.183721
10338003 10.682 0
10338004 9.21055 5.94448e-22
10338005 1.96751 0.983323
10338006 2.26856 0.718439
10338007 2.64433 0.974002
10338008 3.24478 0.965929
10338009 8.45853 0.453583
10338010 2.02489 0.71889
10338011 5.50006 0.866651
10338012 2.14032 0.803918
10338013 1.80753 0.688491
10338014 1.87271 0.995864
10338015 1.84526 0.844515
10338016 7.58745 0.75252
10338017 14.3806 2.76584e-36
10338018 6.73523 0.405112
10338019 4.85623 0.454194
10338020 8.48484 0.966723

Total number of rows: 241576

Table truncated, full table size 6296 Kbytes.




Supplementary file Size Download File type/resource
GSM1224728_WTa1.CEL.gz 3.6 Mb (ftp)(http) CEL
GSM1224728_WTa1.chp.gz 2.2 Mb (ftp)(http) CHP
Processed data included within Sample table
Processed data provided as supplementary file

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