|
| Status |
Public on Aug 31, 2016 |
| Title |
Beta-catenin KLS34 REP3 |
| Sample type |
RNA |
| |
|
| Source name |
sorted KLSCD34-
|
| Organism |
Mus musculus |
| Characteristics |
tissue: Bone marrow
cell type: sorted KLSCD34-
genotype/variation: Beta-catenin
|
| Extracted molecule |
total RNA |
| Extraction protocol |
KLSCD34- stem cells were sorted on a FACS Aria (BD Biosciences) into buffer RLT (Qiagen) containing 2-ME and total RNA was extracted by trizol.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 100ng total RNA.
|
| |
|
| Hybridization protocol |
Following fragmentation, 2ug of cRNA were hybridized for 16 hr at 45C on mouse 1.0 ST Gene Array. Hybridization was performed using an Affymetrix FS450 hybridization oven. GeneChips were washed and stained in the Affymetrix Fluidics Station FS450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G with AGCC.
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
|
| |
|
| Submission date |
Sep 05, 2013 |
| Last update date |
Aug 31, 2016 |
| Contact name |
William Lento |
| Organization name |
Duke University
|
| Department |
Medicine/Cellular Therapy
|
| Lab |
Nelson Chao MD
|
| Street address |
213 Research Drive
|
| City |
Durham |
| State/province |
NC |
| ZIP/Postal code |
27710 |
| Country |
USA |
| |
|
| Platform ID |
GPL10740 |
| Series (1) |
| GSE50620 |
Loss of beta-catenin triggers oxidative stress and impairs hematopoietic regeneration |
|
