|
| Status |
Public on Sep 05, 2013 |
| Title |
rota seizure 3 acute |
| Sample type |
RNA |
| |
|
| Source name |
rotavirus seizure patient 3 in acute phase
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: whole blood
age: 0 years old
gender: male
individual: seizure patient
infection: rotavirus gastroenteritis
disease state: acute
|
| Treatment protocol |
No treatment performed.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Extraction with PAXgene Blood RNA tubes (PreAnalytiX) was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA.
|
| |
|
| Hybridization protocol |
Following fragmentation, 1 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome U133 Plus 2.0 Arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the GeneChip Scanner 3000 and GCOS.
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
|
| |
|
| Submission date |
Sep 05, 2013 |
| Last update date |
Nov 30, 2020 |
| Contact name |
Mitsuru Tsuge |
| E-mail(s) |
powd4x0t@cc.okayama-u.ac.jp
|
| Organization name |
Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
|
| Department |
Pediatrics
|
| Street address |
2-5-1, Shikata-cho
|
| City |
Okayama |
| ZIP/Postal code |
700-8558 |
| Country |
Japan |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE50628 |
Gene expression analysis in children with complex seizures by influenza A (H1N1)pdm09 or rotavirus gastroenteritis |
|
| Relations |
| Reanalyzed by |
GSE162329 |
