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Sample GSM1226043 Query DataSets for GSM1226043
Status Public on Jan 01, 2014
Title WT wounded skin 24hr
Sample type RNA
 
Source name WT_wounded_skin
Organism Mus musculus
Characteristics strain background: C57BL6
genotype/variation: control WT; MED1 fl/fl
age: 8 wks
hair cycling stage: telogen
tissue: skin
sample group: wounded
time after wound: 24 hr
Treatment protocol Med1 KO mice and their littermate control mice received two full-thickness wounds with a 6-mm biopsy punch on the telogen skin. Med1 KO mice showed both telogen and pigmented anagen skin because of hair cycling defects, In contrast, control skin was in telogen in whole area at 8 wk of age. We made wounds on telogen skin. The telogen wounded skin was excised by using 8-mm biopsy punch after 24 hr of wound preparation. Non-wounded telogen skin was also excised from the same mouse as a control.
Growth protocol Coactivator MED1 is removed from keratin 14 (K14) expressing keratinocytes by using Crelox stragety
Extracted molecule total RNA
Extraction protocol Total RNA was isolated from wounded or non wounded skin by using combination of RNAzol and RNAeasy RNA purification kit (Qiagen).
Label biotin
Label protocol Standard Illumina hybridization protocol cDNA labeled by Biotin and detected by Cy3-streptoavidin
 
Hybridization protocol Standard Illumina hybridization protocol done by UCLA neuroscience genomics core
Scan protocol Standard Illumina hybridication protocol done by UCLA neuroscience genomics core
Description WT wound 24hr
Data processing data were normalized by using quantile method in Genome studio V2011.1
 
Submission date Sep 06, 2013
Last update date Jan 01, 2014
Contact name Yuko Oda
E-mail(s) yuko.oda@ucsf.edu
Phone 4152692370
Organization name VA Medical Center UCSF
Department Endocrine Research
Street address 111N-MB 360 1700 Owens St, Suite 360
City San Francisco
State/province CA
ZIP/Postal code 94158
Country USA
 
Platform ID GPL6885
Series (2)
GSE50670 Ablation of coactivator Med1 regulates bulge keratinocyte stem cells and accelerates epidermal regeneration after injury [skin wound]
GSE50673 Ablation of coactivator Med1 regulates bulge keratinocyte stem cells and accelerates epidermal regeneration after injury.

Data table header descriptions
ID_REF
VALUE quantile normalized using Genome Studio
p-value

Data table
ID_REF VALUE p-value
ILMN_1248788 264.4 0.03008
ILMN_2707227 322.5 0.00125
ILMN_2896528 6717.4 0
ILMN_2721178 1576.9 0
ILMN_1227723 1243.9 0
ILMN_3033922 2220.8 0
ILMN_3092673 11404.8 0
ILMN_2730714 8985.9 0
ILMN_3162224 3895.9 0
ILMN_2816356 280.8 0.01504
ILMN_2808939 2866.2 0
ILMN_2634564 1385.2 0
ILMN_1216623 1496.3 0
ILMN_1215157 1960.6 0
ILMN_1228777 187.3 0.46115
ILMN_2737647 207.5 0.21554
ILMN_1216425 176.8 0.67794
ILMN_2734484 1022.5 0
ILMN_2652961 172.9 0.76065
ILMN_2952292 407.5 0

Total number of rows: 25697

Table truncated, full table size 612 Kbytes.




Supplementary data files not provided
Raw data are available on Series record
Processed data included within Sample table

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